Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2378402..2379040 Replicon chromosome
Accession NZ_CP060899
Organism Escherichia coli strain EC21

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag H9206_RS11595 Protein ID WP_000813794.1
Coordinates 2378864..2379040 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag H9206_RS11590 Protein ID WP_001270286.1
Coordinates 2378402..2378818 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9206_RS11570 (2373554) 2373554..2374495 - 942 WP_001251315.1 ABC transporter permease -
H9206_RS11575 (2374496) 2374496..2375509 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
H9206_RS11580 (2375527) 2375527..2376672 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
H9206_RS11585 (2376917) 2376917..2378323 - 1407 WP_000760592.1 PLP-dependent aminotransferase family protein -
H9206_RS11590 (2378402) 2378402..2378818 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
H9206_RS11595 (2378864) 2378864..2379040 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
H9206_RS11600 (2379262) 2379262..2379492 + 231 WP_000494244.1 YncJ family protein -
H9206_RS11605 (2379584) 2379584..2381545 - 1962 WP_001593323.1 23S rRNA 5-hydroxycytidine C2501 synthase -
H9206_RS11610 (2381618) 2381618..2382154 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
H9206_RS11615 (2382246) 2382246..2383421 + 1176 WP_001236215.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T171388 WP_000813794.1 NZ_CP060899:c2379040-2378864 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T171388 NZ_CP077911:c2269288-2269079 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGATTATTAATATAATAGACGGTCAGGTAACTGTAGCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT171388 WP_001270286.1 NZ_CP060899:c2378818-2378402 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT171388 NZ_CP077911:c2269593-2269300 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References