Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 846581..847235 Replicon chromosome
Accession NZ_CP060899
Organism Escherichia coli strain EC21

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag H9206_RS04150 Protein ID WP_000244772.1
Coordinates 846828..847235 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H9206_RS04145 Protein ID WP_000354046.1
Coordinates 846581..846847 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9206_RS04120 (841869) 841869..842612 + 744 WP_000951964.1 SDR family oxidoreductase -
H9206_RS04125 (842669) 842669..844102 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
H9206_RS04130 (844147) 844147..844458 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
H9206_RS04135 (844622) 844622..845281 + 660 WP_000250274.1 hemolysin III family protein -
H9206_RS04140 (845358) 845358..846338 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
H9206_RS04145 (846581) 846581..846847 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H9206_RS04150 (846828) 846828..847235 + 408 WP_000244772.1 protein YgfX Toxin
H9206_RS04155 (847275) 847275..847796 - 522 WP_001055874.1 flavodoxin FldB -
H9206_RS04160 (847908) 847908..848804 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H9206_RS04165 (848829) 848829..849539 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H9206_RS04170 (849545) 849545..851278 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T171375 WP_000244772.1 NZ_CP060899:846828-847235 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T171375 NZ_CP077908:c1126667-1126491 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT171375 WP_000354046.1 NZ_CP060899:846581-846847 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT171375 NZ_CP077908:1126368-1126423 [Staphylococcus aureus]
AAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References