Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 273240..273871 Replicon chromosome
Accession NZ_CP060899
Organism Escherichia coli strain EC21

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag H9206_RS01245 Protein ID WP_001259388.1
Coordinates 273240..273515 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag H9206_RS01250 Protein ID WP_000593555.1
Coordinates 273512..273871 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9206_RS01230 (268244) 268244..269311 + 1068 WP_000361485.1 HlyD family secretion protein -
H9206_RS01235 (269308) 269308..272043 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
H9206_RS01240 (272043) 272043..273167 + 1125 WP_001314210.1 ABC transporter permease -
H9206_RS01245 (273240) 273240..273515 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
H9206_RS01250 (273512) 273512..273871 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H9206_RS01255 (273991) 273991..274392 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
H9206_RS01260 (274398) 274398..275204 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
H9206_RS01265 (275201) 275201..275965 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
H9206_RS01270 (275965) 275965..276798 - 834 WP_038999760.1 nickel ABC transporter permease subunit NikC -
H9206_RS01275 (276795) 276795..277739 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T171373 WP_001259388.1 NZ_CP060899:273240-273515 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T171373 NZ_CP077899:c2609312-2609205 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT171373 WP_000593555.1 NZ_CP060899:273512-273871 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT171373 NZ_CP077899:2609128-2609188 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References