Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 51360..51885 Replicon plasmid pEC22-3
Accession NZ_CP060894
Organism Escherichia coli strain EC22

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag H9207_RS24660 Protein ID WP_001159871.1
Coordinates 51580..51885 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag H9207_RS24655 Protein ID WP_000813634.1
Coordinates 51360..51578 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9207_RS24630 (H9207_24420) 46691..46981 + 291 WP_000648897.1 hypothetical protein -
H9207_RS24635 (H9207_24425) 47554..48555 + 1002 WP_000785695.1 DUF4238 domain-containing protein -
H9207_RS24640 (H9207_24430) 48729..49073 - 345 WP_000792769.1 hypothetical protein -
H9207_RS24645 (H9207_24435) 49571..49825 - 255 WP_000678528.1 hypothetical protein -
H9207_RS24650 (H9207_24440) 49875..50801 - 927 WP_001290414.1 hypothetical protein -
H9207_RS24655 (H9207_24445) 51360..51578 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H9207_RS24660 (H9207_24450) 51580..51885 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
H9207_RS24665 (H9207_24455) 51886..52695 + 810 WP_000016963.1 site-specific integrase -
H9207_RS24670 (H9207_24460) 52867..53061 + 195 Protein_66 colicin M immunity protein -
H9207_RS24675 (H9207_24465) 53117..53814 + 698 WP_223200906.1 IS1-like element IS1A family transposase -
H9207_RS24680 (H9207_24470) 53830..54420 - 591 Protein_68 colicin-like bacteriocin tRNase domain-containing protein -
H9207_RS24685 (H9207_24475) 54600..55026 + 427 Protein_69 tyrosine-type recombinase/integrase -
H9207_RS24690 (H9207_24480) 55218..55493 - 276 WP_000239529.1 hypothetical protein -
H9207_RS24695 (H9207_24485) 55487..56131 - 645 WP_000633913.1 division plane positioning ATPase MipZ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid erm(B) / mph(A) / cmlA1 / qacE / blaCTX-M-14 - 1..110689 110689


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T171372 WP_001159871.1 NZ_CP060894:51580-51885 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T171372 NZ_CP077899:c2268900-2268691 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAAAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT171372 WP_000813634.1 NZ_CP060894:51360-51578 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT171372 NZ_CP077899:c2269205-2268912 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References