Detailed information of TA system TA171371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 30827..31386
• Replicon: plasmid pEC22-3
• Accession: NZ_CP060894
• Organism: Escherichia coli strain EC22

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: H9207_RS24535
• Protein ID: WP_000421257.1
• Coordinates: 31111..31386 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: H9207_RS24530
• Protein ID: WP_001178089.1
• Coordinates: 30827..31111 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9207_RS24505 (H9207_24295)	26502..27074	+	573	Protein_33	IS1380-like element ISEcp1 family transposase	-
H9207_RS24510 (H9207_24300)	27131..28099	-	969	WP_000654812.1	IS5-like element IS903B family transposase	-
H9207_RS24515 (H9207_24305)	28134..29009	-	876	WP_001617865.1	extended-spectrum class A beta-lactamase CTX-M-14	-
H9207_RS24520 (H9207_24310)	29259..29546	-	288	Protein_36	transposase	-
H9207_RS24525 (H9207_24315)	29612..30316	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
H9207_RS24530 (H9207_24320)	30827..31111	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
H9207_RS24535 (H9207_24325)	31111..31386	+	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H9207_RS24545 (H9207_24335)	32198..32566	+	369	Protein_41	colicin-like pore-forming protein	-
H9207_RS24550 (H9207_24340)	32588..32923	-	336	WP_001080730.1	colicin 1A immunity protein	-
H9207_RS24555 (H9207_24345)	33052..33399	+	348	WP_000142451.1	DUF6404 family protein	-
H9207_RS24560 (H9207_24350)	33486..34190	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
H9207_RS24565 (H9207_24355)	34215..34739	+	525	WP_050195267.1	recombinase family protein	-
H9207_RS24570 (H9207_24360)	34816..35478	+	663	WP_241407651.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / mph(A) / cmlA1 / qacE / blaCTX-M-14	-	1..110689	110689
	-	inside	IScluster/Tn	erm(B) / mph(A) / cmlA1 / qacE / blaCTX-M-14	-	10243..42258	32015

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T171371 WP_000421257.1 NZ_CP060894:31111-31386 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T171371 NZ_CP077899:c2249310-2248948 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT171371 WP_001178089.1 NZ_CP060894:30827-31111 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT171371 NZ_CP077899:c2249477-2249307 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: S1PMX5

Antitoxin

• AlphaFold DB: A0A731IHA8