171370

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 44072..44673 Replicon plasmid pEC22-NDM-5
Accession NZ_CP060893
Organism Escherichia coli strain EC22

Toxin (Protein)


Gene name doc Uniprot ID A0A5U0JPI4
Locus tag H9207_RS23615 Protein ID WP_024166788.1
Coordinates 44072..44452 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A5T4YUW9
Locus tag H9207_RS23620 Protein ID WP_033545779.1
Coordinates 44452..44673 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9207_RS23600 (H9207_23375) 40747..41689 + 943 Protein_21 IS3 family transposase -
H9207_RS23605 (H9207_23380) 41716..42412 - 697 Protein_22 IS1 family transposase -
H9207_RS23610 (H9207_23385) 42806..43834 + 1029 WP_072851570.1 tyrosine-type recombinase/integrase -
H9207_RS23615 (H9207_23390) 44072..44452 - 381 WP_024166788.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
H9207_RS23620 (H9207_23395) 44452..44673 - 222 WP_033545779.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
H9207_RS23625 (H9207_23400) 44746..45138 - 393 WP_000506718.1 S24 family peptidase -
H9207_RS23630 (H9207_23405) 45250..45498 - 249 WP_001286530.1 DNA polymerase III subunit theta -
H9207_RS23635 (H9207_23410) 45501..45767 - 267 WP_000946114.1 hypothetical protein -
H9207_RS23640 (H9207_23415) 45837..46127 - 291 WP_048659782.1 hypothetical protein -
H9207_RS23645 (H9207_23425) 46255..46959 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
H9207_RS23650 (H9207_23430) 47012..49225 + 2214 Protein_31 Tn3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / blaTEM-1B / dfrA14 / qnrS1 / floR / blaNDM-5 - 1..168221 168221
- inside IScluster/Tn - - 40747..49225 8478


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(4-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13663.58 Da        Isoelectric Point: 5.6406

>T171370 WP_024166788.1 NZ_CP060893:c44452-44072 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVVYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELFGSNI

Download         Length: 381 bp

>T171370 NZ_CP077899:c2126250-2126074 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGCGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTATAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8147.13 Da        Isoelectric Point: 4.7708

>AT171370 WP_033545779.1 NZ_CP060893:c44673-44452 [Escherichia coli]
MQSINFRTARGNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT171370 NZ_CP077899:2125952-2126006 [Staphylococcus aureus]
AAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5U0JPI4

Antitoxin

Source ID Structure
AlphaFold DB A0A5T4YUW9

References