Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4125358..4125934 Replicon chromosome
Accession NZ_CP060892
Organism Escherichia coli strain EC22

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag H9207_RS19915 Protein ID WP_001297643.1
Coordinates 4125647..4125934 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag H9207_RS19910 Protein ID WP_000063160.1
Coordinates 4125358..4125660 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9207_RS19895 (4121954) 4121954..4124104 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
H9207_RS19900 (4124154) 4124154..4124357 + 204 WP_000467859.1 YbdD/YjiX family protein -
H9207_RS19905 (4124368) 4124368..4125324 + 957 WP_001297640.1 GTPase -
H9207_RS19910 (4125358) 4125358..4125660 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
H9207_RS19915 (4125647) 4125647..4125934 - 288 WP_001297643.1 BrnT family toxin Toxin
H9207_RS25325 (4126133) 4126133..4126829 - 697 Protein_3921 IS1 family transposase -
H9207_RS19930 (4127144) 4127144..4128376 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
H9207_RS19935 (4128417) 4128417..4129697 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4126133..4126342 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T171367 WP_001297643.1 NZ_CP060892:c4125934-4125647 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T171367 NZ_CP077899:1010733-1010837 [Staphylococcus aureus]
CTGTTACTCCTAGAAAGGAATAGCATGTCTGATTTTGAAATGCTAATGGTTGTATTAACGATCATCGGTTTAGTATTAAT
CAGTAATCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT171367 WP_000063160.1 NZ_CP060892:c4125660-4125358 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT171367 NZ_CP077899:c1010959-1010866 [Staphylococcus aureus]
ATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTGGCTTTTCATAGAAAAA
TTATAAAAATAACC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References