Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3497355..3497973 Replicon chromosome
Accession NZ_CP060892
Organism Escherichia coli strain EC22

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H9207_RS16890 Protein ID WP_001291435.1
Coordinates 3497755..3497973 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H9207_RS16885 Protein ID WP_000344800.1
Coordinates 3497355..3497729 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9207_RS16875 (3492444) 3492444..3493637 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H9207_RS16880 (3493660) 3493660..3496809 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
H9207_RS16885 (3497355) 3497355..3497729 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H9207_RS16890 (3497755) 3497755..3497973 + 219 WP_001291435.1 HHA domain-containing protein Toxin
H9207_RS16895 (3498145) 3498145..3498696 + 552 WP_000102564.1 maltose O-acetyltransferase -
H9207_RS16900 (3498812) 3498812..3499282 + 471 WP_000136192.1 YlaC family protein -
H9207_RS16905 (3499446) 3499446..3500996 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H9207_RS16910 (3501038) 3501038..3501391 - 354 WP_000878140.1 DUF1428 domain-containing protein -
H9207_RS16920 (3501770) 3501770..3502081 + 312 WP_000409911.1 MGMT family protein -
H9207_RS16925 (3502112) 3502112..3502684 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T171364 WP_001291435.1 NZ_CP060892:3497755-3497973 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T171364 NZ_CP077898:c2066306-2066097 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAAAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT171364 WP_000344800.1 NZ_CP060892:3497355-3497729 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT171364 NZ_CP077898:c2066611-2066318 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAAAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CATTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References