Detailed information of TA system TA171363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2465936..2466574
• Replicon: chromosome
• Accession: NZ_CP060892
• Organism: Escherichia coli strain EC22

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: H9207_RS11880
• Protein ID: WP_001447010.1
• Coordinates: 2466398..2466574 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H9207_RS11875
• Protein ID: WP_001270286.1
• Coordinates: 2465936..2466352 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9207_RS11855 (2461088)	2461088..2462029	-	942	WP_001251304.1	ABC transporter permease	-
H9207_RS11860 (2462030)	2462030..2463043	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
H9207_RS11865 (2463061)	2463061..2464206	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
H9207_RS11870 (2464451)	2464451..2465857	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
H9207_RS11875 (2465936)	2465936..2466352	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
H9207_RS11880 (2466398)	2466398..2466574	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
H9207_RS11885 (2466796)	2466796..2467026	+	231	WP_000494244.1	YncJ family protein	-
H9207_RS11890 (2467118)	2467118..2469079	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
H9207_RS11895 (2469152)	2469152..2469688	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
H9207_RS11900 (2469780)	2469780..2470955	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T171363 WP_001447010.1 NZ_CP060892:c2466574-2466398 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T171363 NZ_CP077898:c2046716-2046354 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT171363 WP_001270286.1 NZ_CP060892:c2466352-2465936 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT171363 NZ_CP077898:c2046883-2046713 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8