Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 65085..65610 Replicon plasmid pEC24-2
Accession NZ_CP060886
Organism Escherichia coli strain EC24

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag H9208_RS23910 Protein ID WP_001159871.1
Coordinates 65085..65390 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag H9208_RS23915 Protein ID WP_000813630.1
Coordinates 65392..65610 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9208_RS23895 (61047) 61047..62213 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
H9208_RS23900 (62801) 62801..63556 - 756 WP_000852146.1 replication initiation protein RepE -
H9208_RS23905 (64278) 64278..65084 - 807 WP_000016970.1 site-specific integrase -
H9208_RS23910 (65085) 65085..65390 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
H9208_RS23915 (65392) 65392..65610 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H9208_RS23920 (66170) 66170..66400 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
H9208_RS23925 (66397) 66397..66813 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
H9208_RS23930 (66888) 66888..68453 + 1566 WP_001128474.1 AAA family ATPase -
H9208_RS23935 (68438) 68438..69460 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaOXA-1 / aac(6')-Ib-cr / tet(A) / sitABCD iutA / iucD / iucC / iucB / iucA 1..88670 88670


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T171340 WP_001159871.1 NZ_CP060886:c65390-65085 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T171340 NZ_CP077893:613545-613721 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT171340 WP_000813630.1 NZ_CP060886:c65610-65392 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT171340 NZ_CP077893:c613883-613709 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References