Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4365537..4366034 Replicon chromosome
Accession NZ_CP060885
Organism Escherichia coli strain EC24

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H9208_RS21250 Protein ID WP_001173343.1
Coordinates 4365537..4365710 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag H9208_RS21255 Protein ID WP_001425728.1
Coordinates 4365738..4366034 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9208_RS21240 (4362207) 4362207..4363664 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
H9208_RS21245 (4363901) 4363901..4365418 + 1518 WP_001295074.1 lysine--tRNA ligase -
H9208_RS21250 (4365537) 4365537..4365710 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H9208_RS21255 (4365738) 4365738..4366034 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H9208_RS21260 (4366261) 4366261..4366533 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
H9208_RS21265 (4366545) 4366545..4366775 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
H9208_RS21270 (4366956) 4366956..4368587 + 1632 WP_001216487.1 sensor histidine kinase -
H9208_RS21275 (4368584) 4368584..4369303 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T171335 WP_001173343.1 NZ_CP060885:c4365710-4365537 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T171335 NZ_CP077893:149793-149900 [Staphylococcus aureus]
ATGTTCAATTTATTAATTAACATCATGACTTCAGCTATAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT171335 WP_001425728.1 NZ_CP060885:c4366034-4365738 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT171335 NZ_CP077893:c149977-149917 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References