Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 348121..348921 Replicon chromosome
Accession NZ_CP060885
Organism Escherichia coli strain EC24

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag H9208_RS01610 Protein ID WP_000342449.1
Coordinates 348394..348921 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag H9208_RS01605 Protein ID WP_001277108.1
Coordinates 348121..348387 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9208_RS01585 (343778) 343778..344446 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
H9208_RS01590 (344439) 344439..345497 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
H9208_RS01595 (345742) 345742..346596 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
H9208_RS01600 (346868) 346868..347971 + 1104 WP_157297531.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
H9208_RS01605 (348121) 348121..348387 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
H9208_RS01610 (348394) 348394..348921 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
H9208_RS01615 (348918) 348918..349301 - 384 WP_087526099.1 aspartate 1-decarboxylase autocleavage activator PanM -
H9208_RS01620 (349725) 349725..350834 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
H9208_RS01625 (350882) 350882..351808 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
H9208_RS01630 (351805) 351805..353082 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
H9208_RS01635 (353079) 353079..353846 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T171316 WP_000342449.1 NZ_CP060885:348394-348921 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T171316 NZ_CP077885:c2265829-2265563 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT171316 WP_001277108.1 NZ_CP060885:348121-348387 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT171316 NZ_CP077885:c2266086-2265829 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References