171312

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 15278..15803 Replicon plasmid pEC26-3
Accession NZ_CP060882
Organism Escherichia coli strain EC26

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag H9209_RS24090 Protein ID WP_001159871.1
Coordinates 15498..15803 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag H9209_RS24085 Protein ID WP_000813634.1
Coordinates 15278..15496 (+) Length 73 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9209_RS24065 (10440) 10440..11198 + 759 WP_000494510.1 hypothetical protein -
H9209_RS24070 (11489) 11489..13012 + 1524 WP_000921251.1 hypothetical protein -
H9209_RS24075 (13047) 13047..14171 + 1125 WP_000386705.1 DUF4238 domain-containing protein -
H9209_RS24080 (14171) 14171..14461 + 291 WP_000648897.1 hypothetical protein -
H9209_RS24085 (15278) 15278..15496 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H9209_RS24090 (15498) 15498..15803 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
H9209_RS24095 (15804) 15804..16613 + 810 WP_000016963.1 site-specific integrase -
H9209_RS24100 (16785) 16785..17138 + 354 WP_000864813.1 colicin M immunity protein -
H9209_RS24105 (17188) 17188..17757 - 570 Protein_20 lipid II-degrading bacteriocin colicin M -
H9209_RS24110 (17814) 17814..18511 + 698 WP_240763292.1 IS1 family transposase -
H9209_RS24115 (18669) 18669..19541 - 873 WP_000794337.1 hypothetical protein -
H9209_RS24120 (19772) 19772..20469 + 698 WP_223200906.1 IS1-like element IS1A family transposase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..79683 79683


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-100)

Antitoxin

(2-72)


Sequences


Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T171312 WP_001159871.1 NZ_CP060882:15498-15803 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T171312 NZ_CP077885:c1886665-1886456 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT171312 WP_000813634.1 NZ_CP060882:15278-15496 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT171312 NZ_CP077885:c1886970-1886677 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A829CCE8


Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References