Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 15278..15803 Replicon plasmid pEC26-3
Accession NZ_CP060882
Organism Escherichia coli strain EC26

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag H9209_RS24090 Protein ID WP_001159871.1
Coordinates 15498..15803 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag H9209_RS24085 Protein ID WP_000813634.1
Coordinates 15278..15496 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9209_RS24065 (10440) 10440..11198 + 759 WP_000494510.1 hypothetical protein -
H9209_RS24070 (11489) 11489..13012 + 1524 WP_000921251.1 hypothetical protein -
H9209_RS24075 (13047) 13047..14171 + 1125 WP_000386705.1 DUF4238 domain-containing protein -
H9209_RS24080 (14171) 14171..14461 + 291 WP_000648897.1 hypothetical protein -
H9209_RS24085 (15278) 15278..15496 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H9209_RS24090 (15498) 15498..15803 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
H9209_RS24095 (15804) 15804..16613 + 810 WP_000016963.1 site-specific integrase -
H9209_RS24100 (16785) 16785..17138 + 354 WP_000864813.1 colicin M immunity protein -
H9209_RS24105 (17188) 17188..17757 - 570 Protein_20 lipid II-degrading bacteriocin colicin M -
H9209_RS24110 (17814) 17814..18511 + 698 WP_240763292.1 IS1 family transposase -
H9209_RS24115 (18669) 18669..19541 - 873 WP_000794337.1 hypothetical protein -
H9209_RS24120 (19772) 19772..20469 + 698 WP_223200906.1 IS1-like element IS1A family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..79683 79683


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T171312 WP_001159871.1 NZ_CP060882:15498-15803 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T171312 NZ_CP077885:c1886665-1886456 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT171312 WP_000813634.1 NZ_CP060882:15278-15496 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT171312 NZ_CP077885:c1886970-1886677 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References