Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4114808..4115384 Replicon chromosome
Accession NZ_CP060880
Organism Escherichia coli strain EC26

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag H9209_RS20010 Protein ID WP_001297643.1
Coordinates 4115097..4115384 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag H9209_RS20005 Protein ID WP_000063156.1
Coordinates 4114808..4115110 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9209_RS19990 (4111404) 4111404..4113554 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
H9209_RS19995 (4113604) 4113604..4113807 + 204 WP_000467859.1 YbdD/YjiX family protein -
H9209_RS20000 (4113818) 4113818..4114774 + 957 WP_001297640.1 GTPase -
H9209_RS20005 (4114808) 4114808..4115110 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
H9209_RS20010 (4115097) 4115097..4115384 - 288 WP_001297643.1 BrnT family toxin Toxin
H9209_RS20015 (4115583) 4115583..4116280 - 698 Protein_3933 IS1 family transposase -
H9209_RS20020 (4116337) 4116337..4116432 + 96 Protein_3934 dienelactone hydrolase family protein -
H9209_RS20025 (4116578) 4116578..4117810 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
H9209_RS20030 (4117851) 4117851..4119131 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T171309 WP_001297643.1 NZ_CP060880:c4115384-4115097 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T171309 NZ_CP077885:1604098-1604193 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT171309 WP_000063156.1 NZ_CP060880:c4115110-4114808 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT171309 NZ_CP077885:c1604280-1604221 [Staphylococcus aureus]
ACAACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References