Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1866912..1867743 Replicon chromosome
Accession NZ_CP060880
Organism Escherichia coli strain EC26

Toxin (Protein)


Gene name yeeV Uniprot ID -
Locus tag H9209_RS08915 Protein ID WP_039268420.1
Coordinates 1866912..1867286 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID F4THW5
Locus tag H9209_RS08920 Protein ID WP_001313071.1
Coordinates 1867375..1867743 (-) Length 123 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9209_RS08875 (1862308) 1862308..1863474 + 1167 WP_001310935.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
H9209_RS08880 (1863593) 1863593..1864066 + 474 WP_001105393.1 DNA gyrase inhibitor SbmC -
H9209_RS08885 (1864264) 1864264..1865322 + 1059 WP_001200905.1 FUSC family protein -
H9209_RS08890 (1865494) 1865494..1865823 + 330 WP_000450409.1 DUF496 family protein -
H9209_RS08895 (1865924) 1865924..1866058 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
H9209_RS08900 (1866160) 1866160..1866306 + 147 Protein_1746 transposase domain-containing protein -
H9209_RS08905 (1866595) 1866595..1866675 - 81 Protein_1747 hypothetical protein -
H9209_RS08910 (1866721) 1866721..1866915 - 195 WP_000988600.1 DUF5983 family protein -
H9209_RS08915 (1866912) 1866912..1867286 - 375 WP_039268420.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
H9209_RS08920 (1867375) 1867375..1867743 - 369 WP_001313071.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
H9209_RS08925 (1867823) 1867823..1868044 - 222 WP_000692301.1 DUF987 domain-containing protein -
H9209_RS08930 (1868107) 1868107..1868583 - 477 WP_001186726.1 RadC family protein -
H9209_RS08935 (1868599) 1868599..1869078 - 480 WP_000860076.1 antirestriction protein -
H9209_RS08940 (1869160) 1869160..1869981 - 822 WP_001234530.1 DUF932 domain-containing protein -
H9209_RS08945 (1870202) 1870202..1870534 - 333 Protein_1755 hypothetical protein -
H9209_RS08955 (1871380) 1871380..1871568 + 189 Protein_1757 IS4 family transposase -
H9209_RS08960 (1871547) 1871547..1871720 + 174 Protein_1758 cobalamin biosynthesis protein -
H9209_RS08965 (1871872) 1871872..1872417 + 546 WP_000973176.1 bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(18-117)


Sequences


Toxin        


Download         Length: 125 a.a.        Molecular weight: 14000.00 Da        Isoelectric Point: 7.1333

>T171294 WP_039268420.1 NZ_CP060880:c1867286-1866912 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINNIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T171294 NZ_CP077877:1772111-1772287 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13498.23 Da        Isoelectric Point: 5.8696

>AT171294 WP_001313071.1 NZ_CP060880:c1867743-1867375 [Escherichia coli]
VSDTLSGTTHPDDNNSHPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT171294 NZ_CP077877:c1772449-1772275 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A1M1L2Z3

References