Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 89055..89698 Replicon plasmid pEC27-2
Accession NZ_CP060874
Organism Escherichia coli strain EC27

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag H9210_RS23805 Protein ID WP_001034044.1
Coordinates 89282..89698 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag H9210_RS23800 Protein ID WP_001261286.1
Coordinates 89055..89285 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9210_RS23780 (85686) 85686..86441 - 756 WP_000852146.1 replication initiation protein RepE -
H9210_RS23785 (87163) 87163..87969 - 807 WP_000016970.1 site-specific integrase -
H9210_RS23790 (87970) 87970..88275 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
H9210_RS23795 (88277) 88277..88495 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
H9210_RS23800 (89055) 89055..89285 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
H9210_RS23805 (89282) 89282..89698 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
H9210_RS23810 (89773) 89773..91338 + 1566 WP_001128474.1 AAA family ATPase -
H9210_RS23815 (91323) 91323..92345 + 1023 WP_000361402.1 helicase UvrD -
H9210_RS23820 (92599) 92599..93285 - 687 Protein_110 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A) / sitABCD senB / iutA / iucD / iucC / iucB / iucA 1..111544 111544
- flank IS/Tn - - 92599..92946 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T171282 WP_001034044.1 NZ_CP060874:89282-89698 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T171282 NZ_CP077872:2459202-2459468 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT171282 WP_001261286.1 NZ_CP060874:89055-89285 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT171282 NZ_CP077872:2458945-2459202 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References