Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 87970..88495 Replicon plasmid pEC27-2
Accession NZ_CP060874
Organism Escherichia coli strain EC27

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag H9210_RS23790 Protein ID WP_001159871.1
Coordinates 87970..88275 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag H9210_RS23795 Protein ID WP_000813630.1
Coordinates 88277..88495 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9210_RS23775 (83932) 83932..85098 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
H9210_RS23780 (85686) 85686..86441 - 756 WP_000852146.1 replication initiation protein RepE -
H9210_RS23785 (87163) 87163..87969 - 807 WP_000016970.1 site-specific integrase -
H9210_RS23790 (87970) 87970..88275 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
H9210_RS23795 (88277) 88277..88495 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H9210_RS23800 (89055) 89055..89285 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
H9210_RS23805 (89282) 89282..89698 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
H9210_RS23810 (89773) 89773..91338 + 1566 WP_001128474.1 AAA family ATPase -
H9210_RS23815 (91323) 91323..92345 + 1023 WP_000361402.1 helicase UvrD -
H9210_RS23820 (92599) 92599..93285 - 687 Protein_110 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A) / sitABCD senB / iutA / iucD / iucC / iucB / iucA 1..111544 111544
- flank IS/Tn - - 92599..92946 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T171281 WP_001159871.1 NZ_CP060874:c88275-87970 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T171281 NZ_CP077872:1295708-1295896 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTAGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT171281 WP_000813630.1 NZ_CP060874:c88495-88277 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT171281 NZ_CP077872:1295419-1295679 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References