Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4316897..4317394 Replicon chromosome
Accession NZ_CP060873
Organism Escherichia coli strain EC27

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H9210_RS20995 Protein ID WP_001173343.1
Coordinates 4316897..4317070 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag H9210_RS21000 Protein ID WP_001425728.1
Coordinates 4317098..4317394 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9210_RS20985 (4313567) 4313567..4315024 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
H9210_RS20990 (4315261) 4315261..4316778 + 1518 WP_001295074.1 lysine--tRNA ligase -
H9210_RS20995 (4316897) 4316897..4317070 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H9210_RS21000 (4317098) 4317098..4317394 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H9210_RS21005 (4317621) 4317621..4317893 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
H9210_RS21010 (4317905) 4317905..4318135 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
H9210_RS21015 (4318316) 4318316..4319947 + 1632 WP_001216487.1 sensor histidine kinase -
H9210_RS21020 (4319944) 4319944..4320663 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T171276 WP_001173343.1 NZ_CP060873:c4317070-4316897 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T171276 NZ_CP077870:c2572293-2572027 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT171276 WP_001425728.1 NZ_CP060873:c4317394-4317098 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT171276 NZ_CP077870:c2572550-2572293 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References