Detailed information of TA system TA171263

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1350848..1351473
• Replicon: chromosome
• Accession: NZ_CP060873
• Organism: Escherichia coli strain EC27

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H9210_RS06525
• Protein ID: WP_000911330.1
• Coordinates: 1351075..1351473 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: H9210_RS06520
• Protein ID: WP_000450524.1
• Coordinates: 1350848..1351075 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9210_RS06495 (1346651)	1346651..1347121	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
H9210_RS06500 (1347121)	1347121..1347693	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
H9210_RS06505 (1347839)	1347839..1348717	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
H9210_RS06510 (1348734)	1348734..1349768	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
H9210_RS06515 (1349981)	1349981..1350694	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
H9210_RS06520 (1350848)	1350848..1351075	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
H9210_RS06525 (1351075)	1351075..1351473	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
H9210_RS06530 (1351620)	1351620..1352483	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
H9210_RS06535 (1352498)	1352498..1354513	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
H9210_RS06540 (1354587)	1354587..1355285	+	699	WP_000679823.1	esterase	-
H9210_RS06545 (1355395)	1355395..1355595	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T171263 WP_000911330.1 NZ_CP060873:1351075-1351473 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T171263 NZ_CP077869:c1869372-1869163 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCATATGAAACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT171263 WP_000450524.1 NZ_CP060873:1350848-1351075 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT171263 NZ_CP077869:c1869677-1869384 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33