Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 346785..347585 Replicon chromosome
Accession NZ_CP060873
Organism Escherichia coli strain EC27

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag H9210_RS01600 Protein ID WP_000342449.1
Coordinates 347058..347585 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag H9210_RS01595 Protein ID WP_001277108.1
Coordinates 346785..347051 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9210_RS01575 (342442) 342442..343110 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
H9210_RS01580 (343103) 343103..344161 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
H9210_RS01585 (344406) 344406..345260 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
H9210_RS01590 (345532) 345532..346635 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
H9210_RS01595 (346785) 346785..347051 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
H9210_RS01600 (347058) 347058..347585 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
H9210_RS01605 (347582) 347582..347965 - 384 WP_087526099.1 aspartate 1-decarboxylase autocleavage activator PanM -
H9210_RS01610 (348389) 348389..349498 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
H9210_RS01615 (349546) 349546..350472 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
H9210_RS01620 (350469) 350469..351746 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
H9210_RS01625 (351743) 351743..352510 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T171257 WP_000342449.1 NZ_CP060873:347058-347585 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T171257 NZ_CP077868:2107195-2107383 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATATTCGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT171257 WP_001277108.1 NZ_CP060873:346785-347051 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT171257 NZ_CP077868:2106906-2107166 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References