Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 325494..326080 Replicon chromosome
Accession NZ_CP060873
Organism Escherichia coli strain EC27

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag H9210_RS01475 Protein ID WP_042014162.1
Coordinates 325494..325724 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag H9210_RS01480 Protein ID WP_000593555.1
Coordinates 325721..326080 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9210_RS01465 (321531) 321531..324266 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
H9210_RS01470 (324266) 324266..325390 + 1125 WP_001314210.1 ABC transporter permease -
H9210_RS01475 (325494) 325494..325724 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
H9210_RS01480 (325721) 325721..326080 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H9210_RS01485 (326200) 326200..326601 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
H9210_RS01490 (326607) 326607..327413 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
H9210_RS01495 (327410) 327410..328174 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
H9210_RS01500 (328174) 328174..329007 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
H9210_RS01505 (329004) 329004..329948 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T171256 WP_042014162.1 NZ_CP060873:325494-325724 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T171256 NZ_CP077868:c2089476-2089156 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACATGTGTACTTAAAAGAATTCAGTGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCGTCATCAGAGAACTTTGACGAGGAATCAGATGACGCAAAGAATTCAATAAAAGTAGCAGAGTA
A

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT171256 WP_000593555.1 NZ_CP060873:325721-326080 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT171256 NZ_CP077868:c2089688-2089479 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACTTGGTTTATCAACTTGAGTTAAGTGTCATGAATAATTTGGAAAGTTA
CGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References