Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 133539..134164 Replicon plasmid pEC29-NDM-5
Accession NZ_CP060868
Organism Escherichia coli strain EC29

Toxin (Protein)


Gene name vapC Uniprot ID A0A1D7QA53
Locus tag H9211_RS24420 Protein ID WP_039023147.1
Coordinates 133539..133937 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1D7QA08
Locus tag H9211_RS24425 Protein ID WP_039023146.1
Coordinates 133937..134164 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9211_RS24405 (129774) 129774..130271 + 498 WP_000605862.1 entry exclusion protein -
H9211_RS24410 (130303) 130303..131034 + 732 WP_000850422.1 conjugal transfer complement resistance protein TraT -
H9211_RS24415 (131287) 131287..133530 + 2244 WP_094907434.1 type IV conjugative transfer system coupling protein TraD -
H9211_RS24420 (133539) 133539..133937 - 399 WP_039023147.1 type II toxin-antitoxin system VapC family toxin Toxin
H9211_RS24425 (133937) 133937..134164 - 228 WP_039023146.1 toxin-antitoxin system antitoxin VapB Antitoxin

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / mph(A) - 1..147459 147459


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-43)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14885.26 Da        Isoelectric Point: 8.2824

>T171250 WP_039023147.1 NZ_CP060868:c133937-133539 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAAIHT
GQIRAELARQGRPVGPFDQMIAGHARCRGLIIVTNNTREFERVGGLRIEDWS

Download         Length: 399 bp

>T171250 NZ_CP077868:525334-525600 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGAAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGTTATTATAGTAGACGTATCAATCACGAACATAGATTGGTCTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8549.62 Da        Isoelectric Point: 4.7513

>AT171250 WP_039023146.1 NZ_CP060868:c134164-133937 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAIGRTRLITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Download         Length: 228 bp

>AT171250 NZ_CP077868:525083-525334 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACCTCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAATCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGAAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D7QA53

Antitoxin

Source ID Structure
AlphaFold DB A0A1D7QA08

References