Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 71035..71560 Replicon plasmid pEC29-NDM-5
Accession NZ_CP060868
Organism Escherichia coli strain EC29

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag H9211_RS24060 Protein ID WP_001159871.1
Coordinates 71255..71560 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag H9211_RS24055 Protein ID WP_000813630.1
Coordinates 71035..71253 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9211_RS24030 (66245) 66245..66931 + 687 Protein_78 IS1 family transposase -
H9211_RS24035 (67185) 67185..68207 - 1023 WP_000361402.1 helicase UvrD -
H9211_RS24040 (68192) 68192..69757 - 1566 WP_001128474.1 AAA family ATPase -
H9211_RS24045 (69832) 69832..70248 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
H9211_RS24050 (70245) 70245..70475 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
H9211_RS24055 (71035) 71035..71253 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H9211_RS24060 (71255) 71255..71560 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
H9211_RS24065 (71561) 71561..72367 + 807 WP_000016970.1 site-specific integrase -
H9211_RS24070 (73089) 73089..73844 + 756 WP_000852146.1 replication initiation protein RepE -
H9211_RS24075 (74432) 74432..75598 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / mph(A) - 1..147459 147459


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T171246 WP_001159871.1 NZ_CP060868:71255-71560 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T171246 NZ_CP077867:2332405-2332614 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCATATGAAACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT171246 WP_000813630.1 NZ_CP060868:71035-71253 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT171246 NZ_CP077867:2332100-2332393 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References