171245

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 69832..70475 Replicon plasmid pEC29-NDM-5
Accession NZ_CP060868
Organism Escherichia coli strain EC29

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag H9211_RS24045 Protein ID WP_001034044.1
Coordinates 69832..70248 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag H9211_RS24050 Protein ID WP_001261286.1
Coordinates 70245..70475 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9211_RS24020 (65085) 65085..65198 - 114 Protein_76 IS6 family transposase -
H9211_RS24025 (65398) 65398..66095 + 698 WP_223350503.1 IS1 family transposase -
H9211_RS24030 (66245) 66245..66931 + 687 Protein_78 IS1 family transposase -
H9211_RS24035 (67185) 67185..68207 - 1023 WP_000361402.1 helicase UvrD -
H9211_RS24040 (68192) 68192..69757 - 1566 WP_001128474.1 AAA family ATPase -
H9211_RS24045 (69832) 69832..70248 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
H9211_RS24050 (70245) 70245..70475 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
H9211_RS24055 (71035) 71035..71253 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
H9211_RS24060 (71255) 71255..71560 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
H9211_RS24065 (71561) 71561..72367 + 807 WP_000016970.1 site-specific integrase -
H9211_RS24070 (73089) 73089..73844 + 756 WP_000852146.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / mph(A) - 1..147459 147459


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T171245 WP_001034044.1 NZ_CP060868:c70248-69832 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T171245 NZ_CP077867:2272513-2272617 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT171245 WP_001261286.1 NZ_CP060868:c70475-70245 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT171245 NZ_CP077867:c2272776-2272612 [Staphylococcus aureus]
GTAGTAAGTAGAAGCAAAAGATGAAAATCTTTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAAATGCCGGTCAAAGCGAATAGAAGGT
TATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References