Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3744590..3745244 Replicon chromosome
Accession NZ_CP060867
Organism Escherichia coli strain EC29

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag H9211_RS18060 Protein ID WP_000244777.1
Coordinates 3744590..3744997 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H9211_RS18065 Protein ID WP_000354046.1
Coordinates 3744978..3745244 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9211_RS18040 (3740547) 3740547..3742280 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
H9211_RS18045 (3742286) 3742286..3742996 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H9211_RS18050 (3743021) 3743021..3743917 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H9211_RS18055 (3744029) 3744029..3744550 + 522 WP_001055874.1 flavodoxin FldB -
H9211_RS18060 (3744590) 3744590..3744997 - 408 WP_000244777.1 protein YgfX Toxin
H9211_RS18065 (3744978) 3744978..3745244 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H9211_RS18070 (3745487) 3745487..3746467 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
H9211_RS18075 (3746663) 3746663..3747322 - 660 WP_000250274.1 hemolysin III family protein -
H9211_RS18080 (3747486) 3747486..3747797 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
H9211_RS18085 (3747842) 3747842..3749275 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
H9211_RS18090 (3749332) 3749332..3750075 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T171240 WP_000244777.1 NZ_CP060867:c3744997-3744590 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T171240 NZ_CP077867:10792-10968 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT171240 WP_000354046.1 NZ_CP060867:c3745244-3744978 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT171240 NZ_CP077867:c11130-10956 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References