Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2768414..2769249 Replicon chromosome
Accession NZ_CP060867
Organism Escherichia coli strain EC29

Toxin (Protein)


Gene name yeeV Uniprot ID A0A1U9SHU4
Locus tag H9211_RS13480 Protein ID WP_000854752.1
Coordinates 2768872..2769249 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID E3PJ72
Locus tag H9211_RS13475 Protein ID WP_001278232.1
Coordinates 2768414..2768782 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9211_RS13450 (2764416) 2764416..2764889 + 474 WP_000855059.1 antirestriction protein -
H9211_RS13455 (2764975) 2764975..2765721 - 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
H9211_RS13460 (2765736) 2765736..2767277 - 1542 WP_002431311.1 IS21-like element ISEc12 family transposase -
H9211_RS13465 (2767491) 2767491..2767967 + 477 WP_001186774.1 RadC family protein -
H9211_RS13470 (2768030) 2768030..2768251 + 222 WP_000692345.1 DUF987 domain-containing protein -
H9211_RS13475 (2768414) 2768414..2768782 + 369 WP_001278232.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
H9211_RS13480 (2768872) 2768872..2769249 + 378 WP_000854752.1 TA system toxin CbtA family protein Toxin
H9211_RS13485 (2769246) 2769246..2769395 + 150 Protein_2643 DUF5983 family protein -
H9211_RS13490 (2769495) 2769495..2769575 + 81 Protein_2644 hypothetical protein -
H9211_RS13495 (2770373) 2770373..2770762 + 390 WP_001445118.1 IS110 family transposase -
H9211_RS13500 (2771033) 2771033..2771398 + 366 WP_223350516.1 EutP/PduV family microcompartment system protein -
H9211_RS13505 (2771499) 2771499..2771828 - 330 WP_000450409.1 DUF496 family protein -
H9211_RS13510 (2772000) 2772000..2773058 - 1059 WP_001200892.1 FUSC family protein -
H9211_RS13515 (2773256) 2773256..2773729 - 474 WP_001105384.1 DNA gyrase inhibitor SbmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14076.08 Da        Isoelectric Point: 8.2905

>T171237 WP_000854752.1 NZ_CP060867:2768872-2769249 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp

>T171237 NZ_CP077865:c1057700-1057593 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13491.37 Da        Isoelectric Point: 6.6290

>AT171237 WP_001278232.1 NZ_CP060867:2768414-2768782 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT171237 NZ_CP077865:1057516-1057576 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1U9SHU4

Antitoxin

Source ID Structure
AlphaFold DB E3PJ72

References