Detailed information of TA system TA171227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2206461..2207099
• Replicon: chromosome
• Accession: NZ_CP060867
• Organism: Escherichia coli strain EC29

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: H9211_RS10725
• Protein ID: WP_001447010.1
• Coordinates: 2206461..2206637 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H9211_RS10730
• Protein ID: WP_001270286.1
• Coordinates: 2206683..2207099 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9211_RS10705 (2202080)	2202080..2203255	-	1176	WP_001236265.1	BenE family transporter YdcO	-
H9211_RS10710 (2203347)	2203347..2203883	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
H9211_RS10715 (2203956)	2203956..2205917	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
H9211_RS10720 (2206009)	2206009..2206239	-	231	WP_000494244.1	YncJ family protein	-
H9211_RS10725 (2206461)	2206461..2206637	+	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
H9211_RS10730 (2206683)	2206683..2207099	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
H9211_RS10735 (2207178)	2207178..2208584	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
H9211_RS10740 (2208829)	2208829..2209974	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
H9211_RS10745 (2209992)	2209992..2211005	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
H9211_RS10750 (2211006)	2211006..2211947	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T171227 WP_001447010.1 NZ_CP060867:2206461-2206637 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T171227 NZ_CP077863:2175704-2175913 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCATATGAAACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT171227 WP_001270286.1 NZ_CP060867:2206683-2207099 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT171227 NZ_CP077863:2175399-2175692 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8