Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 546604..547180 Replicon chromosome
Accession NZ_CP060867
Organism Escherichia coli strain EC29

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag H9211_RS02630 Protein ID WP_001297643.1
Coordinates 546604..546891 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag H9211_RS02635 Protein ID WP_000063160.1
Coordinates 546878..547180 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9211_RS02615 (542841) 542841..544121 - 1281 WP_001037390.1 DUF445 domain-containing protein -
H9211_RS02620 (544162) 544162..545394 - 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
H9211_RS02625 (545709) 545709..546405 + 697 Protein_511 IS1 family transposase -
H9211_RS02630 (546604) 546604..546891 + 288 WP_001297643.1 BrnT family toxin Toxin
H9211_RS02635 (546878) 546878..547180 + 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
H9211_RS02640 (547214) 547214..548170 - 957 WP_001297640.1 GTPase -
H9211_RS02645 (548181) 548181..548384 - 204 WP_000467859.1 YbdD/YjiX family protein -
H9211_RS02650 (548434) 548434..550584 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 546196..546405 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T171223 WP_001297643.1 NZ_CP060867:546604-546891 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T171223 NZ_CP077863:678882-679070 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATATTCGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT171223 WP_000063160.1 NZ_CP060867:546878-547180 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT171223 NZ_CP077863:678593-678853 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References