Detailed information of TA system TA171218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 47596..48203
• Replicon: plasmid pAML0157.2
• Accession: NZ_CP060863
• Organism: Enterococcus faecium strain AML0157

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: IA041_RS14650
• Protein ID: WP_002321032.1
• Coordinates: 47853..48203 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: IA041_RS14645
• Protein ID: WP_002287514.1
• Coordinates: 47596..47859 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IA041_RS14605	42632..43531	+	900	WP_002322787.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
IA041_RS14610	43534..44019	+	486	WP_002296241.1	single-stranded DNA-binding protein	-
IA041_RS14615	44385..44645	+	261	WP_002296240.1	hypothetical protein	-
IA041_RS14620	45086..45292	+	207	WP_002296239.1	hypothetical protein	-
IA041_RS14625	45292..45543	+	252	WP_002296238.1	hypothetical protein	-
IA041_RS14630	45558..45995	+	438	WP_002296237.1	hypothetical protein	-
IA041_RS14635	45988..46695	+	708	WP_002353391.1	hypothetical protein	-
IA041_RS14640	47076..47255	+	180	WP_002305761.1	hypothetical protein	-
IA041_RS14645	47596..47859	+	264	WP_002287514.1	hypothetical protein	Antitoxin
IA041_RS14650	47853..48203	+	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IA041_RS14655	48227..48841	-	615	WP_010729795.1	recombinase family protein	-
IA041_RS14660	49155..49577	+	423	WP_002288787.1	hypothetical protein	-
IA041_RS14665	49587..49790	+	204	WP_002299573.1	hypothetical protein	-
IA041_RS14670	50001..50585	+	585	WP_002299575.1	recombinase family protein	-
IA041_RS14675	50774..51454	-	681	WP_010861579.1	IS6-like element IS1216 family transposase	-
IA041_RS14680	51508..52218	-	711	WP_010729797.1	GntR family transcriptional regulator	-
IA041_RS14685	52202..52936	-	735	WP_010729798.1	GntR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..261677	261677

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T171218 WP_002321032.1 NZ_CP060863:47853-48203 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T171218 NZ_CP077861:c1015291-1015025 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGAAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGTTATTATAGTAGACGTATCAATCACGAACATAGATTGGTCTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT171218 WP_002287514.1 NZ_CP060863:47596-47859 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT171218 NZ_CP077861:c1015542-1015291 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACCTCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAATCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGAAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0