Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 25430..26567 Replicon plasmid pAML0157.1
Accession NZ_CP060862
Organism Enterococcus faecium strain AML0157

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag IA041_RS14275 Protein ID WP_002332783.1
Coordinates 25430..26293 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag IA041_RS14280 Protein ID WP_002326825.1
Coordinates 26295..26567 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IA041_RS14230 20848..21582 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
IA041_RS14235 21563..21814 - 252 Protein_26 nucleotidyltransferase domain-containing protein -
IA041_RS14240 21889..22569 + 681 WP_001067799.1 IS6-like element IS1216 family transposase -
IA041_RS14245 22624..22815 + 192 Protein_28 recombinase family protein -
IA041_RS14250 22958..23182 + 225 WP_000205227.1 helix-turn-helix transcriptional regulator -
IA041_RS14255 23197..23823 + 627 Protein_30 nucleotidyltransferase domain-containing protein -
IA041_RS14260 23877..24557 - 681 WP_001067799.1 IS6-like element IS1216 family transposase -
IA041_RS14265 24632..24712 - 81 Protein_32 IS6 family transposase -
IA041_RS14270 24787..24867 - 81 Protein_33 IS6 family transposase -
IA041_RS14275 25430..26293 - 864 WP_002332783.1 toxin zeta Toxin
IA041_RS14280 26295..26567 - 273 WP_002326825.1 antitoxin Antitoxin
IA041_RS14285 26585..26794 - 210 WP_000527318.1 peptide-binding protein -
IA041_RS14290 26892..27788 - 897 WP_002326827.1 ParA family protein -
IA041_RS14300 29310..29570 - 261 Protein_39 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
IA041_RS14305 29740..30477 - 738 WP_001038795.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
IA041_RS14310 30602..30700 - 99 WP_176698583.1 23S rRNA methyltransferase attenuation leader peptide -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ant(6)-Ia / erm(B) / cat(pC221) / VanHAX - 1..49122 49122
- inside IScluster/Tn ant(6)-Ia / erm(B) / cat(pC221) - 13849..35862 22013


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T171217 WP_002332783.1 NZ_CP060862:c26293-25430 [Enterococcus faecium]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T171217 NZ_CP077861:c753740-753636 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT171217 WP_002326825.1 NZ_CP060862:c26567-26295 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp

>AT171217 NZ_CP077861:753542-753580 [Staphylococcus aureus]
AACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTGAC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References