Detailed information of TA system TA171217

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 25430..26567
• Replicon: plasmid pAML0157.1
• Accession: NZ_CP060862
• Organism: Enterococcus faecium strain AML0157

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: P0A4M2
• Locus tag: IA041_RS14275
• Protein ID: WP_002332783.1
• Coordinates: 25430..26293 (-)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: Q9AL19
• Locus tag: IA041_RS14280
• Protein ID: WP_002326825.1
• Coordinates: 26295..26567 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IA041_RS14230	20848..21582	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
IA041_RS14235	21563..21814	-	252	Protein_26	nucleotidyltransferase domain-containing protein	-
IA041_RS14240	21889..22569	+	681	WP_001067799.1	IS6-like element IS1216 family transposase	-
IA041_RS14245	22624..22815	+	192	Protein_28	recombinase family protein	-
IA041_RS14250	22958..23182	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	-
IA041_RS14255	23197..23823	+	627	Protein_30	nucleotidyltransferase domain-containing protein	-
IA041_RS14260	23877..24557	-	681	WP_001067799.1	IS6-like element IS1216 family transposase	-
IA041_RS14265	24632..24712	-	81	Protein_32	IS6 family transposase	-
IA041_RS14270	24787..24867	-	81	Protein_33	IS6 family transposase	-
IA041_RS14275	25430..26293	-	864	WP_002332783.1	toxin zeta	Toxin
IA041_RS14280	26295..26567	-	273	WP_002326825.1	antitoxin	Antitoxin
IA041_RS14285	26585..26794	-	210	WP_000527318.1	peptide-binding protein	-
IA041_RS14290	26892..27788	-	897	WP_002326827.1	ParA family protein	-
IA041_RS14300	29310..29570	-	261	Protein_39	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
IA041_RS14305	29740..30477	-	738	WP_001038795.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
IA041_RS14310	30602..30700	-	99	WP_176698583.1	23S rRNA methyltransferase attenuation leader peptide	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	ant(6)-Ia / erm(B) / cat(pC221) / VanHAX	-	1..49122	49122
	-	inside	IScluster/Tn	ant(6)-Ia / erm(B) / cat(pC221)	-	13849..35862	22013

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32675.27 Da
• Isoelectric Point: 7.3939

>T171217 WP_002332783.1 NZ_CP060862:c26293-25430 [Enterococcus faecium]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Nucleotide

• Length: 864 bp

>T171217 NZ_CP077861:c753740-753636 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10720.17 Da
• Isoelectric Point: 4.4865

>AT171217 WP_002326825.1 NZ_CP060862:c26567-26295 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Nucleotide

• Length: 273 bp

>AT171217 NZ_CP077861:753542-753580 [Staphylococcus aureus]
AACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTGAC

Structures

Toxin

• AlphaFold DB: P0A4M2

Antitoxin

• AlphaFold DB: A0A0M2AF93