Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 498443..499014 Replicon chromosome
Accession NZ_CP060861
Organism Enterococcus faecium strain AML0157

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag IA041_RS02375 Protein ID WP_002286801.1
Coordinates 498673..499014 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag IA041_RS02370 Protein ID WP_002323011.1
Coordinates 498443..498673 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IA041_RS02340 493579..494907 + 1329 WP_008265691.1 FAD-containing oxidoreductase -
IA041_RS02345 494929..495555 + 627 WP_002286816.1 cysteine hydrolase -
IA041_RS02350 495738..496319 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
IA041_RS02355 497051..497398 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
IA041_RS02360 497374..497613 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
IA041_RS02365 497818..498156 - 339 WP_002286804.1 hypothetical protein -
IA041_RS02370 498443..498673 + 231 WP_002323011.1 hypothetical protein Antitoxin
IA041_RS02375 498673..499014 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
IA041_RS02380 499864..500049 + 186 WP_002304207.1 hypothetical protein -
IA041_RS02385 500554..500748 + 195 WP_002297028.1 hypothetical protein -
IA041_RS02390 500906..501188 + 283 Protein_474 transposase -
IA041_RS02395 501432..502262 - 831 WP_002286789.1 manganese catalase family protein -
IA041_RS02400 502470..503804 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T171216 WP_002286801.1 NZ_CP060861:498673-499014 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T171216 NZ_CP077861:c693848-693639 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCATATGAAACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT171216 WP_002323011.1 NZ_CP060861:498443-498673 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT171216 NZ_CP077861:c694153-693860 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References