Detailed information of TA system TA171066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1007402..1008216
• Replicon: chromosome
• Accession: NZ_CP060834
• Organism: Salmonella enterica strain 1505

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A8E5IG67
• Locus tag: IAR36_RS04800
• Protein ID: WP_000971651.1
• Coordinates: 1007402..1007929 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: M7S6I2
• Locus tag: IAR36_RS04805
• Protein ID: WP_000855694.1
• Coordinates: 1007926..1008216 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAR36_RS04770 (1003676)	1003676..1004074	+	399	Protein_937	cytoplasmic protein	-
IAR36_RS04775 (1004265)	1004265..1004504	+	240	Protein_938	hypothetical protein	-
IAR36_RS04780 (1004661)	1004661..1005329	+	669	WP_000445914.1	hypothetical protein	-
IAR36_RS04785 (1005356)	1005356..1005850	+	495	WP_020899151.1	hypothetical protein	-
IAR36_RS04790 (1006022)	1006022..1006678	-	657	WP_023181885.1	protein-serine/threonine phosphatase	-
IAR36_RS04795 (1006911)	1006911..1007329	+	419	Protein_942	transposase	-
IAR36_RS04800 (1007402)	1007402..1007929	-	528	WP_000971651.1	GNAT family N-acetyltransferase	Toxin
IAR36_RS04805 (1007926)	1007926..1008216	-	291	WP_000855694.1	DUF1778 domain-containing protein	Antitoxin
IAR36_RS04810 (1008810)	1008810..1009136	+	327	WP_000393295.1	hypothetical protein	-
IAR36_RS04815 (1009409)	1009409..1009756	-	348	WP_001555786.1	DUF1493 family protein	-
IAR36_RS04820 (1009741)	1009741..1010190	-	450	WP_133322517.1	hypothetical protein	-
IAR36_RS04825 (1010622)	1010622..1011065	-	444	WP_000715096.1	SPI-1 type III secretion system invasion lipoprotein InvH	-
IAR36_RS04830 (1011522)	1011522..1012172	+	651	WP_001728903.1	type III secretion system transcriptional activator InvF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	invH / invF / invG / invE / invA / invB	1006022..1017485	11463
	-	flank	IS/Tn	-	-	1007153..1007329	176

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 18970.77 Da
• Isoelectric Point: 9.3905

>T171066 WP_000971651.1 NZ_CP060834:c1007929-1007402 [Salmonella enterica]
MMFTDWHEAAIGKTHNRMNFDCGDADLNQFLQRHARQNHEKGTTKTYVALDNSDVTRIHGFYSVSPASLIYAQVPGAISK
GLGGYDVPVFRLGRLAVDKSMQGQGLGAQLLLSAGKRCIQAALQVGGVALLIDAKNKQVCDWYKGFGAVPLNDQPLSLLL
SFKTLYAALSASGRL

Nucleotide

• Length: 528 bp

>T171066 NZ_CP077823:c5179194-5178811 [Klebsiella africana]
ATGACCTGCGGATCTGCGCTTTTTGATACCAACATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCAGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCAAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTAAGCGCTTTTAATATCATCAACATCTCGCAGGATATTGCAGAGCGAAGCGTTGCTCTG
CGGCAGGAGTATAAGCTTAAGCTGCCAGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
AAATACGAGGGATTTTGCCGGCATTCCTGGCGTGGTCACTCCGTACGAACTACACCCTGAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10664.50 Da
• Isoelectric Point: 6.3133

>AT171066 WP_000855694.1 NZ_CP060834:c1008216-1007926 [Salmonella enterica]
MKTMPQIAIESNERLSLRVSTDAKKLIVRAAAIQQTNLTDFVVSNVLPVAQKIVDAAERVYLTERDTQMIMEILDNPPAP
NEKLLAAAFALPDMKK

Nucleotide

• Length: 291 bp

>AT171066 NZ_CP077823:c5179436-5179194 [Klebsiella africana]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTTAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCAAGCTAAAGATA
TCATCTCCAGTGCGCTGGGCCTGTGGCAAGACTGCGAAGAAGATGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A3V8SJE7