Detailed information of TA system TA171059

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 175751..176658
• Replicon: plasmid pLs
• Accession: NZ_CP060819
• Organism: Pantoea ananatis strain Lstri

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: IAQ00_RS22875
• Protein ID: WP_089528392.1
• Coordinates: 176191..176658 (+)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A8A4JZ23
• Locus tag: IAQ00_RS22870
• Protein ID: WP_014598335.1
• Coordinates: 175751..176194 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAQ00_RS22840 (IAQ00_22770)	170890..171276	-	387	WP_013027868.1	pyrimidine utilization protein C	-
IAQ00_RS22845 (IAQ00_22775)	171302..172054	-	753	WP_028722713.1	pyrimidine utilization protein B	-
IAQ00_RS22850 (IAQ00_22780)	172051..173142	-	1092	WP_206116316.1	pyrimidine utilization protein A	-
IAQ00_RS22855 (IAQ00_22785)	173541..174230	+	690	WP_014598333.1	HTH-type transcriptional regulator RutR	-
IAQ00_RS22860 (IAQ00_22790)	174306..175001	-	696	WP_252295078.1	hypothetical protein	-
IAQ00_RS22865 (IAQ00_22795)	175141..175365	-	225	WP_013027863.1	KTSC domain-containing protein	-
IAQ00_RS22870 (IAQ00_22800)	175751..176194	+	444	WP_014598335.1	DUF2384 domain-containing protein	Antitoxin
IAQ00_RS22875 (IAQ00_22805)	176191..176658	+	468	WP_089528392.1	RES family NAD+ phosphorylase	Toxin
IAQ00_RS22880 (IAQ00_22810)	177303..178697	+	1395	WP_047713815.1	flagellar filament capping protein FliD	-
IAQ00_RS22885 (IAQ00_22815)	179380..180315	+	936	WP_028722485.1	flagellin	-
IAQ00_RS22890 (IAQ00_22820)	180377..180949	-	573	WP_014598338.1	NADPH-dependent FMN reductase	-
IAQ00_RS22895 (IAQ00_22825)	181037..181270	+	234	WP_072208328.1	DUF333 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	vipA/tssB / vipB/tssC / fliC	1..267898	267898

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (2-145)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (31-89)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17190.69 Da
• Isoelectric Point: 5.1568

>T171059 WP_089528392.1 NZ_CP060819:176191-176658 [Pantoea ananatis]
MKLYRMTKTRYLGSAWSGFGAREGGGRWNSVGVSMVYASETASLTMLETLIHLQSASVLDCFTLMSIDVPDRLIEWIDIK
QLPDDWAAPEAPAALRLFGDAWIQSGGSVALRVPSALSPVESNYLLNPEHPEFNAIVRQAVNIPFLFDVRFSRQA

Nucleotide

• Length: 468 bp

>T171059 NZ_CP077823:c1764581-1764228 [Klebsiella africana]
GTGTGGACGATTAAAACAACGGATATGTTTGATCGCTGGTTTACCTCGCTCAATGATATCGATCGGGCCAGCGTGCTGGC
AGCGCTGTTGGTCTTGCGGGAAAAGGGGCCGGGTTTGTCCAGACCTTATGCCGATACGATCAGAGGTTCCCGCTACAGCA
ACATGAAAGAGTTGCGTGTTCAGAGCCGGGGCGACCCAATACGGGCGTTTTTTGCTTTTGACCCGGCCCGTACCGGCATT
GTGCTTTGTGCCGGAAATAAGGTCGGCAATGAAAAACGGTTTTACCATGAGATGCTCGCGGTCGCAGATCGGGAATTCAC
GAACTGGCTTAACAGATTGAAAGAGAAGGAGTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16375.69 Da
• Isoelectric Point: 7.0208

>AT171059 WP_014598335.1 NZ_CP060819:175751-176194 [Pantoea ananatis]
MKQFTLPVSAAEPDRLWQFAGLSSADGVMLMDQIDDGLDGTVASRIVTWAQISQAELRRMTGIAGSTFTRGVKSRFTAEQ
SERLVRFIRVMDRAVELFEGDKEKARQWLNEPARALGNKKPADLVASETGAYEVLKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT171059 NZ_CP077823:c1764223-1763921 [Klebsiella africana]
ATGGGCAGAACGCTGGAACAGCTTCTTATAGATGAAAAACCGGATGTCGTTGCCGAAGCTCAGGCTATTGCTAAGGATAT
CCTGCTTAATATTCATCTTGCCGAACTACGTGAGAAGGTGCAGAAGACCCAGGTAGAAATGGCTCAGGATTTAGGCATAA
AACAGCCTACCGTTGCAGGAATGGAAAAACCGGGCCGCGACCTGAAGCTCTCTACCCTTAAGCGTTATGTTGAAGCGGCG
GGTGGTAAGCTGCGGCTTGATGTTGAGCTCCCGGACGGGTCGCACTATGAGTTTGTTTTGTAG