Detailed information of TA system TA171045

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 2823380..2824125
• Replicon: chromosome
• Accession: NZ_CP060817
• Organism: Xanthomonas oryzae pv. oryzae strain C9-3

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: H9N23_RS13120
• Protein ID: WP_199285672.1
• Coordinates: 2823380..2823709 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: H9N23_RS13125
• Protein ID: WP_011407754.1
• Coordinates: 2823706..2824125 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9N23_RS13090 (H9N23_13065)	2818511..2819182	+	672	WP_011407759.1	class I SAM-dependent methyltransferase	-
H9N23_RS13100 (H9N23_13075)	2819484..2820275	-	792	WP_011407758.1	polysaccharide deacetylase family protein	-
H9N23_RS13105 (H9N23_13080)	2820321..2821247	+	927	WP_011257932.1	Grx4 family monothiol glutaredoxin	-
H9N23_RS13110 (H9N23_13085)	2821579..2821914	+	336	WP_011407757.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
H9N23_RS13115 (H9N23_13090)	2821958..2823277	-	1320	WP_109182117.1	IS701-like element ISXo15 family transposase	-
H9N23_RS13120 (H9N23_13095)	2823380..2823709	+	330	WP_199285672.1	DUF4433 domain-containing protein	Toxin
H9N23_RS13125 (H9N23_13100)	2823706..2824125	+	420	WP_011407754.1	macro domain-containing protein	Antitoxin
H9N23_RS13130 (H9N23_13105)	2824177..2825145	+	969	WP_011258802.1	IS5-like element ISXo1 family transposase	-
H9N23_RS13135 (H9N23_13110)	2825280..2825936	+	657	WP_075246166.1	phosphate phosphatase	-
H9N23_RS13140 (H9N23_13115)	2825974..2827209	-	1236	WP_011257570.1	ISL3-like element ISXoo13 family transposase	-
H9N23_RS13145 (H9N23_13120)	2827511..2827837	+	327	Protein_2521	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2821958..2827209	5251

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (3-108)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 13145.86 Da
• Isoelectric Point: 7.1160

>T171045 WP_199285672.1 NZ_CP060817:2823380-2823709 [Xanthomonas oryzae pv. oryzae]
MHHVAERRWPFLFTDSHAYCQLASFYSDLSDLDKIDWPLLQARDFRRDPDDPAKFERYQAEALIHRHLPLDGLRGIVCHT
DGLKQTIERQLQERNLKLPVHTRTDWYFR

Nucleotide

• Length: 330 bp

>T171045 NZ_CP077819:c76469-76053 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15607.02 Da
• Isoelectric Point: 6.4733

>AT171045 WP_011407754.1 NZ_CP060817:2823706-2824125 [Xanthomonas oryzae pv. oryzae]
MITFTQGNLLESGAEALVNTVNTVGVMGKGIALMFKERFKENFLRYAAACKDNQVRIGKIFVTEVNELDGPRWIINFPTK
QHWRGDSRIEWITEGLQDLHRFLIENKVKSIAIPPLGAGNGGLDWAEVRPLIEEVLGNL

Nucleotide

• Length: 420 bp

>AT171045 NZ_CP077819:c76696-76466 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGAGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA