Detailed information of TA system TA171038

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 59313..59901
• Replicon: plasmid pXM9F202-2-186k
• Accession: NZ_CP060812
• Organism: Acinetobacter variabilis strain XM9F202-2

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: IAQ69_RS15430
• Protein ID: WP_086384391.1
• Coordinates: 59313..59600 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: IAQ69_RS15435
• Protein ID: WP_023274838.1
• Coordinates: 59587..59901 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAQ69_RS15410	55151..55855	-	705	WP_200230923.1	IS6 family transposase	-
IAQ69_RS15415	55918..56085	-	168	Protein_45	transposase	-
IAQ69_RS15420	56317..58728	-	2412	WP_032060263.1	membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family	-
IAQ69_RS15425	58746..59030	+	285	Protein_47	hypothetical protein	-
IAQ69_RS15430	59313..59600	+	288	WP_086384391.1	BrnT family toxin	Toxin
IAQ69_RS15435	59587..59901	+	315	WP_023274838.1	BrnA antitoxin family protein	Antitoxin
IAQ69_RS15440	60029..60592	-	564	WP_086384392.1	macro domain-containing protein	-
IAQ69_RS15445	60662..61198	+	537	WP_166139238.1	PD-(D/E)XK nuclease family protein	-
IAQ69_RS15455	62538..62654	+	117	WP_001791010.1	tetracycline resistance determinant leader peptide	-
IAQ69_RS15460	62670..64589	+	1920	WP_000691727.1	tetracycline resistance ribosomal protection protein Tet(M)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(M) / sul2 / aph(3')-Ia / aph(3'')-Ib / aph(6)-Id / aac(3)-IId / msr(E) / mph(E) / floR	-	1..186242	186242

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.67 Da
• Isoelectric Point: 5.2134

>T171038 WP_086384391.1 NZ_CP060812:59313-59600 [Acinetobacter variabilis]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFDDPLRISIQDRHTDGEERWQTIGKVKGVLMMLVAHTIFDEDDCEIIRIIS
ARQVTKAERDRYEHG

Nucleotide

• Length: 288 bp

>T171038 NZ_CP077818:2152409-2153326 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGCGGCACTGCAATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAGTCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12298.98 Da
• Isoelectric Point: 9.4860

>AT171038 WP_023274838.1 NZ_CP060812:59587-59901 [Acinetobacter variabilis]
MSMVRYSRKELNEKFSDKQDAEIQRLLAKGTVPDDQLDLSDMPEITDWSNAVRHNRFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT171038 NZ_CP077818:2151645-2152403 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTTTATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA