Detailed information of TA system TA171035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 253150..253672
• Replicon: plasmid p2757-346
• Accession: NZ_CP060810
• Organism: Klebsiella variicola strain KP2757

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A7H0EVH6
• Locus tag: IAP99_RS28780
• Protein ID: WP_014839901.1
• Coordinates: 253388..253672 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A7H0EVH5
• Locus tag: IAP99_RS28775
• Protein ID: WP_014839900.1
• Coordinates: 253150..253398 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAP99_RS28750	248328..248684	+	357	WP_017901071.1	hypothetical protein	-
IAP99_RS28755	249058..249456	+	399	WP_014839899.1	H-NS histone family protein	-
IAP99_RS28760	249593..250201	+	609	WP_024266306.1	DUF2913 family protein	-
IAP99_RS28765	250745..251377	+	633	WP_001567369.1	hypothetical protein	-
IAP99_RS28770	251406..252809	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
IAP99_RS28775	253150..253398	+	249	WP_014839900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IAP99_RS28780	253388..253672	+	285	WP_014839901.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IAP99_RS28785	253863..254339	+	477	WP_042934526.1	hypothetical protein	-
IAP99_RS28790	254345..255022	+	678	WP_042934501.1	hypothetical protein	-
IAP99_RS28795	255039..255758	-	720	WP_042934502.1	cation:proton antiporter	-
IAP99_RS28800	255999..256601	-	603	WP_014839902.1	hypothetical protein	-
IAP99_RS28805	256598..257119	-	522	WP_048336588.1	hypothetical protein	-
IAP99_RS28810	257200..258144	-	945	WP_014839903.1	DsbA family protein	-
IAP99_RS28815	258207..258416	-	210	WP_125316032.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	fosA3 / sul2 / aph(3'')-Ib / aph(6)-Id / qnrS1 / aac(6')-Ib-cr / ARR-3 / blaTEM-1B / blaCTX-M-3 / dfrA12 / aadA2 / qacE / sul1 / armA / msr(E) / mph(E) / aac(3)-IId / aadA5 / dfrA1	-	1..346514	346514
	-	flank	IS/Tn	-	-	251406..252809	1403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10866.46 Da
• Isoelectric Point: 10.5831

>T171035 WP_014839901.1 NZ_CP060810:253388-253672 [Klebsiella variicola]
MTYKLAFNESALKEWKKLGHTIREQFKKKLSERLQNPRVPAAQLHGGKDQYKIKLHGAGYRLVYSVNDEIVTVTVIGVGK
RNNDDIYNATRHRN

Nucleotide

• Length: 285 bp

>T171035 NZ_CP077816:5040265-5040675 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTAGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9071.29 Da
• Isoelectric Point: 3.8832

>AT171035 WP_014839900.1 NZ_CP060810:253150-253398 [Klebsiella variicola]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMEMLEDQELGRIADERIGEDRIEVNID
DL

Nucleotide

• Length: 249 bp

>AT171035 NZ_CP077816:5040018-5040284 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A7H0EVH6

Antitoxin

• AlphaFold DB: A0A7H0EVH5