Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 41458..42101 Replicon plasmid p2757-138
Accession NZ_CP060808
Organism Klebsiella variicola strain KP2757

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag IAP99_RS26550 Protein ID WP_001044770.1
Coordinates 41458..41874 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag IAP99_RS26555 Protein ID WP_001261282.1
Coordinates 41871..42101 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IAP99_RS26525 36708..37466 + 759 Protein_40 peptide ABC transporter permease SapB -
IAP99_RS26530 37509..38213 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
IAP99_RS26535 38287..38559 - 273 Protein_42 IS1 family transposase -
IAP99_RS26540 38815..39837 - 1023 WP_000361404.1 helicase UvrD -
IAP99_RS26545 39822..41384 - 1563 WP_004206609.1 AAA family ATPase -
IAP99_RS26550 41458..41874 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
IAP99_RS26555 41871..42101 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IAP99_RS26560 42058..42519 + 462 WP_187726404.1 hypothetical protein -
IAP99_RS26565 42680..43624 + 945 WP_011977810.1 hypothetical protein -
IAP99_RS26570 43661..44053 + 393 WP_011977811.1 hypothetical protein -
IAP99_RS26575 44111..44632 + 522 WP_013214008.1 hypothetical protein -
IAP99_RS26580 44678..44881 + 204 WP_011977813.1 hypothetical protein -
IAP99_RS26585 44911..45915 + 1005 WP_011977814.1 hypothetical protein -
IAP99_RS26590 46099..46878 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-14 / sul1 / blaDHA-1 / qnrB4 - 1..138721 138721
- flank IS/Tn - - 37509..38213 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T171033 WP_001044770.1 NZ_CP060808:c41874-41458 [Klebsiella variicola]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T171033 NZ_CP077816:4555244-4555591 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATTCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGAGCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT171033 WP_001261282.1 NZ_CP060808:c42101-41871 [Klebsiella variicola]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT171033 NZ_CP077816:4555591-4555890 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References