Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5380498..5381123 Replicon chromosome
Accession NZ_CP060807
Organism Klebsiella variicola strain KP2757

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag IAP99_RS25960 Protein ID WP_008807903.1
Coordinates 5380498..5380881 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IAP99_RS25965 Protein ID WP_004150355.1
Coordinates 5380881..5381123 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IAP99_RS25945 5377864..5378766 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IAP99_RS25950 5378763..5379398 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
IAP99_RS25955 5379395..5380324 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IAP99_RS25960 5380498..5380881 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
IAP99_RS25965 5380881..5381123 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IAP99_RS25970 5381328..5382245 + 918 WP_064171510.1 alpha/beta hydrolase -
IAP99_RS25975 5382259..5383200 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
IAP99_RS25980 5383245..5383682 - 438 WP_008807906.1 D-tyrosyl-tRNA(Tyr) deacylase -
IAP99_RS25985 5383679..5384539 - 861 WP_008807907.1 virulence factor BrkB family protein -
IAP99_RS25990 5384533..5385132 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T171032 WP_008807903.1 NZ_CP060807:c5380881-5380498 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T171032 NZ_CP077816:4529273-4529641 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGACAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT171032 WP_004150355.1 NZ_CP060807:c5381123-5380881 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT171032 NZ_CP077816:4529055-4529276 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References