Detailed information of TA system TA171031

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 5060290..5061971
• Replicon: chromosome
• Accession: NZ_CP060807
• Organism: Klebsiella variicola strain KP2757

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A1F2M1V2
• Locus tag: IAP99_RS24480
• Protein ID: WP_032734038.1
• Coordinates: 5060290..5061207 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: IAP99_RS24485
• Protein ID: WP_110242892.1
• Coordinates: 5061213..5061971 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAP99_RS24460	5055596..5057116	+	1521	WP_110242893.1	sugar ABC transporter ATP-binding protein	-
IAP99_RS24465	5057113..5058111	+	999	WP_023296904.1	ribose ABC transporter permease	-
IAP99_RS24470	5058142..5059083	+	942	WP_012543155.1	ABC transporter substrate-binding protein	-
IAP99_RS24475	5059158..5060165	+	1008	WP_022065799.1	carbohydrate kinase family protein	-
IAP99_RS24480	5060290..5061207	-	918	WP_032734038.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
IAP99_RS24485	5061213..5061971	-	759	WP_110242892.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
IAP99_RS24490	5062056..5062784	-	729	WP_008807258.1	response regulator transcription factor	-
IAP99_RS24495	5063057..5065204	+	2148	WP_077625453.1	formate dehydrogenase subunit alpha	-
IAP99_RS24500	5065558..5066244	+	687	WP_008807260.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-246)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34733.10 Da
• Isoelectric Point: 5.4707

>T171031 WP_032734038.1 NZ_CP060807:c5061207-5060290 [Klebsiella variicola]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRTIALPHIRDALARIAANLQQQ
AGINAVLQANRSDEMRVVVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELNRPIFNGFIAYLLSHNRPLAEVLSPRWKDIAEPFYREFSGMTFETIALEELTAVPYRMIDALKS
CFTQQDIDFLLSFKRGEPDWRLAPETRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T171031 NZ_CP077816:c4128172-4127789 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28657.94 Da
• Isoelectric Point: 10.1988

>AT171031 WP_110242892.1 NZ_CP060807:c5061971-5061213 [Klebsiella variicola]
MSSKLNWLLQNSAPGDVILQSWLSRHAISPSQAFKYTQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSMENKALLPKWFREFPGVEWIVISGQKLPVLDDKYRVTLDVKGKRLTGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQTKRLYLFFASFYEHGWLRRIDSQKIDLGAGKRQIVVNGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT171031 NZ_CP077816:c4128414-4128172 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A1F2M1V2