Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2751589..2753177 Replicon chromosome
Accession NZ_CP060807
Organism Klebsiella variicola strain KP2757

Toxin (Protein)


Gene name hipA Uniprot ID A0A0B7G636
Locus tag IAP99_RS13455 Protein ID WP_012968244.1
Coordinates 2751589..2752911 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A2V3KHJ7
Locus tag IAP99_RS13460 Protein ID WP_038422544.1
Coordinates 2752911..2753177 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IAP99_RS13440 2746654..2749155 + 2502 WP_110243053.1 fimbrial biogenesis outer membrane usher protein -
IAP99_RS13445 2749143..2750147 + 1005 WP_032730346.1 type 1 fimbrial protein -
IAP99_RS13450 2750280..2751572 + 1293 WP_012968243.1 glycoside hydrolase family 10 protein -
IAP99_RS13455 2751589..2752911 - 1323 WP_012968244.1 type II toxin-antitoxin system HipA family toxin Toxin
IAP99_RS13460 2752911..2753177 - 267 WP_038422544.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
IAP99_RS13465 2753443..2753769 - 327 WP_008804921.1 hydroxyisourate hydrolase -
IAP99_RS13470 2753766..2754266 - 501 WP_008804922.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
IAP99_RS13475 2754244..2755623 - 1380 WP_012968246.1 purine permease -
IAP99_RS13480 2755969..2757123 + 1155 WP_008804924.1 FAD-dependent urate hydroxylase HpxO -
IAP99_RS13485 2757104..2758027 - 924 WP_008804925.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(5-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(13-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 48896.29 Da        Isoelectric Point: 7.9109

>T171028 WP_012968244.1 NZ_CP060807:c2752911-2751589 [Klebsiella variicola]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGGHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMAFLLGSSEALKDRADFMKFMVFQWLVGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPGELMLAILQEFAGHVP
QAIESARRTLPADFSTHVWRAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T171028 NZ_CP077816:c3504887-3504354 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCGAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9923.30 Da        Isoelectric Point: 8.5912

>AT171028 WP_038422544.1 NZ_CP060807:c2753177-2752911 [Klebsiella variicola]
MTSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKATETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT171028 NZ_CP077816:c3505164-3504898 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B7G636

Antitoxin

Source ID Structure
AlphaFold DB A0A2V3KHJ7

References