Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1855214..1855765 Replicon chromosome
Accession NZ_CP060807
Organism Klebsiella variicola strain KP2757

Toxin (Protein)


Gene name pemK Uniprot ID A0A2V3KRZ7
Locus tag IAP99_RS08965 Protein ID WP_046881584.1
Coordinates 1855214..1855546 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A2V3KU66
Locus tag IAP99_RS08970 Protein ID WP_046881585.1
Coordinates 1855547..1855765 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IAP99_RS08935 1850979..1852079 - 1101 WP_070612302.1 phosphotransferase -
IAP99_RS08940 1852606..1852863 + 258 WP_012541132.1 antitoxin -
IAP99_RS08945 1852864..1853196 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin -
IAP99_RS08955 1853519..1854955 + 1437 WP_042948427.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
IAP99_RS08965 1855214..1855546 - 333 WP_046881584.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
IAP99_RS08970 1855547..1855765 - 219 WP_046881585.1 antitoxin Antitoxin
IAP99_RS08975 1855953..1857887 + 1935 WP_046881586.1 NTPase KAP -
IAP99_RS08980 1857887..1858753 + 867 WP_046881587.1 hypothetical protein -
IAP99_RS08985 1858750..1860117 + 1368 WP_072057612.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11751.64 Da        Isoelectric Point: 10.8919

>T171019 WP_046881584.1 NZ_CP060807:c1855546-1855214 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKAAFNKQTRLPVVVPVTSGGNFARATGFTVSLDGVGTKTTGVLRCDQPRTI
VMGARSGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T171019 NZ_CP077815:c51192-50722 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACCAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTAATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCTGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 7877.07 Da        Isoelectric Point: 4.1364

>AT171019 WP_046881585.1 NZ_CP060807:c1855765-1855547 [Klebsiella variicola]
MLAVPPAVLKTLGLSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAVGKEIM

Download         Length: 219 bp

>AT171019 NZ_CP077815:c51632-51189 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTCCTGGGGCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAATGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACGGGAGCGTATGAGG
TCATTAAGCTGATTACACGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V3KRZ7

Antitoxin

Source ID Structure
AlphaFold DB A0A2V3KU66

References