Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1852606..1853196 Replicon chromosome
Accession NZ_CP060807
Organism Klebsiella variicola strain KP2757

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag IAP99_RS08945 Protein ID WP_008804165.1
Coordinates 1852864..1853196 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag IAP99_RS08940 Protein ID WP_012541132.1
Coordinates 1852606..1852863 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IAP99_RS08920 1848214..1848789 + 576 WP_012541129.1 hypothetical protein -
IAP99_RS08925 1848968..1849804 + 837 WP_008804155.1 alpha/beta hydrolase -
IAP99_RS08930 1850011..1850982 + 972 WP_016161433.1 sensor domain-containing diguanylate cyclase -
IAP99_RS08935 1850979..1852079 - 1101 WP_070612302.1 phosphotransferase -
IAP99_RS08940 1852606..1852863 + 258 WP_012541132.1 antitoxin Antitoxin
IAP99_RS08945 1852864..1853196 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
IAP99_RS08955 1853519..1854955 + 1437 WP_042948427.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
IAP99_RS08965 1855214..1855546 - 333 WP_046881584.1 type II toxin-antitoxin system PemK/MazF family toxin -
IAP99_RS08970 1855547..1855765 - 219 WP_046881585.1 antitoxin -
IAP99_RS08975 1855953..1857887 + 1935 WP_046881586.1 NTPase KAP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T171018 WP_008804165.1 NZ_CP060807:1852864-1853196 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T171018 NZ_CP077815:c46378-45893 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTACTTCGTTGCTATCGGGTTGCCGAGAATATAGGTGTACGTGCAATCATGGTTCATGCGCTCACAG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCCGCGGACACTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT171018 WP_012541132.1 NZ_CP060807:1852606-1852863 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT171018 NZ_CP077815:c46632-46366 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References