Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 337377..337963 Replicon chromosome
Accession NZ_CP060807
Organism Klebsiella variicola strain KP2757

Toxin (Protein)


Gene name doc Uniprot ID A0A2W7TCK5
Locus tag IAP99_RS01550 Protein ID WP_008806973.1
Coordinates 337595..337963 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag IAP99_RS01545 Protein ID WP_004174006.1
Coordinates 337377..337598 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IAP99_RS01525 333526..334452 + 927 WP_012540389.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
IAP99_RS01530 334449..335726 + 1278 WP_008806971.1 branched chain amino acid ABC transporter permease LivM -
IAP99_RS01535 335723..336490 + 768 WP_008806972.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
IAP99_RS01540 336492..337205 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
IAP99_RS01545 337377..337598 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IAP99_RS01550 337595..337963 + 369 WP_008806973.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
IAP99_RS01555 338255..339571 + 1317 WP_048268284.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
IAP99_RS01560 339673..340560 + 888 WP_012967120.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
IAP99_RS01565 340557..341402 + 846 WP_048268283.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
IAP99_RS01570 341404..342474 + 1071 WP_187726303.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 334449..343211 8762


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(6-116)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13639.01 Da        Isoelectric Point: 7.3191

>T171015 WP_008806973.1 NZ_CP060807:337595-337963 [Klebsiella variicola]
MTLQIISAEEIIQFHDRLLRVTPDVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLVISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T171015 NZ_CP077814:c4648414-4648312 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT171015 WP_004174006.1 NZ_CP060807:337377-337598 [Klebsiella variicola]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT171015 NZ_CP077814:4648305-4648450 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W7TCK5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References