Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 296543..297174 Replicon chromosome
Accession NZ_CP060807
Organism Klebsiella variicola strain KP2757

Toxin (Protein)


Gene name hicA Uniprot ID A0A2V3KJT3
Locus tag IAP99_RS01340 Protein ID WP_044650325.1
Coordinates 296543..296818 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R4YIG2
Locus tag IAP99_RS01345 Protein ID WP_004181489.1
Coordinates 296815..297174 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IAP99_RS01320 291779..292114 + 336 WP_002921203.1 universal stress protein UspB -
IAP99_RS01325 292174..293670 - 1497 WP_008806938.1 inorganic phosphate transporter PitA -
IAP99_RS01330 293900..295093 + 1194 WP_012967106.1 NAD(P)/FAD-dependent oxidoreductase -
IAP99_RS01335 295133..296146 - 1014 WP_008806942.1 magnesium transporter -
IAP99_RS01340 296543..296818 + 276 WP_044650325.1 type II toxin-antitoxin system HicA family toxin Toxin
IAP99_RS01345 296815..297174 + 360 WP_004181489.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
IAP99_RS01350 297202..297603 - 402 WP_032733900.1 nickel-responsive transcriptional regulator NikR -
IAP99_RS01355 297591..298382 - 792 WP_012540373.1 nickel import ATP-binding protein NikE -
IAP99_RS01360 298379..299143 - 765 WP_008806946.1 nickel import ATP-binding protein NikD -
IAP99_RS01365 299143..299976 - 834 WP_012967107.1 nickel ABC transporter permease subunit NikC -
IAP99_RS01370 299973..300917 - 945 WP_016161924.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10279.91 Da        Isoelectric Point: 10.3165

>T171014 WP_044650325.1 NZ_CP060807:296543-296818 [Klebsiella variicola]
MEQQVLSLRNKQRHTLEQLFKIPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

>T171014 NZ_CP077814:c4162667-4162350 [Klebsiella pneumoniae]
ATGTTGACTTTTATTGAACTACAAGGTTTCAGTAAGCGACGCCAGGTGCTGTTGCAGGATGATGAATTTCGGGCCTTTCA
GGAAATCCTTATAAACGATCCCGAGGCTGGGGACACAATTTCGGGAACCGGTGGTTTTCGTAAAATTCGCTGGAGTCGTC
CCGGGATGGGGAAGCGCGGCGGTGTACGAGTGATTTACTATAATGTAACGAAAAAGGGAAGAATTTACCTGGCGCTCATC
TATCCTAAAAATGAACAGGATGAGTTAAATCAGGAGCAAAAAAAGATGTTGAGACAAGTGGCTGATTTAATAAACTAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13313.02 Da        Isoelectric Point: 4.4605

>AT171014 WP_004181489.1 NZ_CP060807:296815-297174 [Klebsiella variicola]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp

>AT171014 NZ_CP077814:c4162274-4161978 [Klebsiella pneumoniae]
ATGAAAGACGAATTGTTTGCCGATCTGCTGGCGAGTGCAGAAGAAATGGTCAGGATCGAGAAAGGTGAACAAATGCCGAT
GGAGCAGCACGTTCATACCTTCAGCGAAATTGACGTGAAAGCGATACGTGAAGCCACAGGTTTAAAACAGCAGGACTTTG
CAGCGGCCGTTGGCGTTAGCTACGAGTTGGTTAAAAGCTGGGAAACGAAACGACGTCAGCCAACCGGTGCAGCCAGAAAA
CTGCTTTTATTGCTTCAGGGTAATCCATTGATTATCAACTTGTTGAAGGTTGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V3KJT3

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M5Q1

References