Detailed information of TA system TA171013

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 2491979..2492674
• Replicon: chromosome
• Accession: NZ_CP060804
• Organism: Enterococcus faecalis strain SF28073

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: A0A7H0FN33
• Locus tag: H9Q64_RS13435
• Protein ID: WP_010773525.1
• Coordinates: 2492330..2492674 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: H9Q64_RS13430
• Protein ID: WP_002385633.1
• Coordinates: 2491979..2492311 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9Q64_RS13385	2487428..2488207	-	780	WP_010773518.1	DnaD domain protein	-
H9Q64_RS13390	2488222..2489037	-	816	WP_010773519.1	PD-(D/E)XK nuclease-like domain-containing protein	-
H9Q64_RS13395	2489000..2490016	-	1017	WP_002393794.1	recombinase RecT	-
H9Q64_RS13400	2490119..2490343	-	225	WP_002370012.1	hypothetical protein	-
H9Q64_RS13405	2490340..2490639	-	300	WP_010773520.1	hypothetical protein	-
H9Q64_RS13410	2490726..2491142	-	417	WP_010774349.1	hypothetical protein	-
H9Q64_RS13415	2491143..2491364	-	222	WP_025192439.1	hypothetical protein	-
H9Q64_RS13420	2491336..2491479	-	144	WP_010773523.1	hypothetical protein	-
H9Q64_RS13425	2491490..2491666	-	177	WP_010773524.1	helix-turn-helix domain-containing protein	-
H9Q64_RS13430	2491979..2492311	+	333	WP_002385633.1	helix-turn-helix domain-containing protein	Antitoxin
H9Q64_RS13435	2492330..2492674	+	345	WP_010773525.1	ImmA/IrrE family metallo-endopeptidase	Toxin
H9Q64_RS13440	2492777..2494144	+	1368	WP_010773526.1	recombinase family protein	-
H9Q64_RS13445	2494166..2494742	-	577	Protein_2403	DNA repair protein RadC	-
H9Q64_RS13450	2494774..2495433	-	660	WP_002385286.1	HAD family hydrolase	-
H9Q64_RS13455	2495417..2496739	-	1323	WP_002415877.1	bifunctional folylpolyglutamate synthase/dihydrofolate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2451981..2494717	42736

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (21-89)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-63)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13537.40 Da
• Isoelectric Point: 4.9418

>T171013 WP_010773525.1 NZ_CP060804:2492330-2492674 [Enterococcus faecalis]
MKSIKELVEEYNVELVFTTLNKRACFDPTYGIIFVNQNLTPSEQEEAIYHEFKHVKDHADLMALYNIPIFRSKMEAEAEH
YMFECLIEKNDGQFNYSNVITHYNLKMGQETYLK

Nucleotide

• Length: 345 bp

>T171013 NZ_CP077814:3919724-3921046 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGATAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTAGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCGCCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGCCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGATATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12677.13 Da
• Isoelectric Point: 4.6211

>AT171013 WP_002385633.1 NZ_CP060804:2491979-2492311 [Enterococcus faecalis]
MNLLERIKKLAKDRDVSIYQLEEEINIGRNTIYQWNKRTPSSDKLEAVANYFNVSVDYLLGRTENPNQAGAKPSDDLDDV
LDNVMSFDGEPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 333 bp

>AT171013 NZ_CP077814:3919458-3919724 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7H0FN33