Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 6753..7239 Replicon plasmid pSF3
Accession NZ_CP060803
Organism Enterococcus faecalis strain SF28073

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag H9Q64_RS00855 Protein ID WP_001284312.1
Coordinates 6753..6965 (-) Length 71 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag H9Q64_RS00860 Protein ID WP_000301765.1
Coordinates 6967..7239 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9Q64_RS00840 2133..2828 - 696 WP_001280781.1 VanA-type vancomycin resistance DNA-binding response regulator VanR -
H9Q64_RS00845 3042..3617 - 576 WP_001226076.1 recombinase family protein -
H9Q64_RS00850 3763..6729 + 2967 WP_000684987.1 Tn3 family transposase -
H9Q64_RS00855 6753..6965 - 213 WP_001284312.1 zeta toxin family protein Toxin
H9Q64_RS00860 6967..7239 - 273 WP_000301765.1 antitoxin Antitoxin
H9Q64_RS00865 7256..7465 - 210 WP_000527317.1 peptide-binding protein -
H9Q64_RS00870 7563..8459 - 897 WP_001809760.1 ParA family protein -
H9Q64_RS00875 8562..8846 - 285 WP_073459356.1 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
H9Q64_RS00880 8992..9585 - 594 WP_187726547.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
H9Q64_RS00885 9586..10191 - 606 WP_000238804.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..46541 46541


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(26-54)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 71 a.a.        Molecular weight: 7405.42 Da        Isoelectric Point: 7.9915

>T171010 WP_001284312.1 NZ_CP060803:c6965-6753 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELVQGKKAVESPTAFLLGGQPGSGKTSLRSAILGVASGKCGFTTLSLFS

Download         Length: 213 bp

>T171010 NZ_CP077814:1801302-1801586 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCTGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT171010 WP_000301765.1 NZ_CP060803:c7239-6967 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT171010 NZ_CP077814:1801070-1801312 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References