Detailed information of TA system TA171005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-DinJ
• Location: 11430..11964
• Replicon: plasmid pSF2
• Accession: NZ_CP060802
• Organism: Enterococcus faecalis strain SF28073

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R3JX52
• Locus tag: H9Q64_RS00575
• Protein ID: WP_002360769.1
• Coordinates: 11689..11964 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: R3H6P0
• Locus tag: H9Q64_RS00570
• Protein ID: WP_002369771.1
• Coordinates: 11430..11696 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9Q64_RS00535	7288..8073	+	786	WP_002360778.1	replication-relaxation family protein	-
H9Q64_RS00540	8079..8567	+	489	WP_002412099.1	hypothetical protein	-
H9Q64_RS00545	9159..9425	+	267	WP_002360776.1	hypothetical protein	-
H9Q64_RS00550	9458..9958	+	501	WP_002360775.1	DnaJ domain-containing protein	-
H9Q64_RS00555	9971..10219	+	249	WP_002360773.1	DUF3850 domain-containing protein	-
H9Q64_RS00560	10295..10711	+	417	WP_002414801.1	single-stranded DNA-binding protein	-
H9Q64_RS00565	10735..11319	+	585	WP_002383617.1	thermonuclease family protein	-
H9Q64_RS00570	11430..11696	+	267	WP_002369771.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
H9Q64_RS00575	11689..11964	+	276	WP_002360769.1	type II toxin-antitoxin system YafQ family toxin	Toxin
H9Q64_RS00580	12769..12954	+	186	WP_002368099.1	hypothetical protein	-
H9Q64_RS00585	12939..13130	+	192	WP_002383620.1	DpnD/PcfM family protein	-
H9Q64_RS00590	13362..14141	+	780	WP_002383621.1	hypothetical protein	-
H9Q64_RS00595	14141..15400	+	1260	WP_002360763.1	PcfJ domain-containing protein	-
H9Q64_RS00600	16049..16777	+	729	WP_002383622.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-aph(2'') / qacD	-	1..54644	54644

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (7-86)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10732.58 Da
• Isoelectric Point: 9.6918

>T171005 WP_002360769.1 NZ_CP060802:11689-11964 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Nucleotide

• Length: 276 bp

>T171005 NZ_CP077814:c429012-428602 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9772.09 Da
• Isoelectric Point: 4.4791

>AT171005 WP_002369771.1 NZ_CP060802:11430-11696 [Enterococcus faecalis]
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Nucleotide

• Length: 267 bp

>AT171005 NZ_CP077814:c429259-428993 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0M2AEA7

Antitoxin

• AlphaFold DB: A0A0M2AED7