171003

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 70620..71191 Replicon plasmid pSF1
Accession NZ_CP060801
Organism Enterococcus faecalis strain SF28073

Toxin (Protein)


Gene name mazF Uniprot ID R3HR30
Locus tag H9Q64_RS00365 Protein ID WP_001278770.1
Coordinates 70620..70961 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A6B1XRC3
Locus tag H9Q64_RS00370 Protein ID WP_000958277.1
Coordinates 70961..71191 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9Q64_RS00340 66269..66595 + 327 WP_000606299.1 hypothetical protein -
H9Q64_RS00345 67474..67749 - 276 WP_000889655.1 type II toxin-antitoxin system YafQ family toxin -
H9Q64_RS00350 67742..68008 - 267 WP_000246197.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
H9Q64_RS00355 68721..69323 - 603 WP_000892301.1 Fic family protein -
H9Q64_RS00360 69570..70508 - 939 WP_001053446.1 hypothetical protein -
H9Q64_RS00365 70620..70961 - 342 WP_001278770.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
H9Q64_RS00370 70961..71191 - 231 WP_000958277.1 hypothetical protein Antitoxin
H9Q64_RS00375 71421..72026 + 606 WP_000599739.1 cell filamentation protein -
H9Q64_RS00380 72042..72614 + 573 WP_000170424.1 recombinase family protein -
H9Q64_RS00385 73047..74006 - 960 WP_000222577.1 IS30-like element IS1252 family transposase -
H9Q64_RS00390 74134..74814 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
H9Q64_RS00395 75137..75307 + 171 WP_000713595.1 hypothetical protein -
H9Q64_RS00400 75321..75923 + 603 WP_001062586.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ant(6)-Ia / aph(3')-III / erm(B) prgB/asc10 1..96486 96486
- flank IS/Tn - - 64943..66121 1178


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13220.44 Da        Isoelectric Point: 8.8595

>T171003 WP_001278770.1 NZ_CP060801:c70961-70620 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T171003 NZ_CP077812:4330999-4331325 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8536.45 Da        Isoelectric Point: 4.2412

>AT171003 WP_000958277.1 NZ_CP060801:c71191-70961 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWYDIEPGGRELF

Download         Length: 231 bp

>AT171003 NZ_CP077812:4331346-4331645 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACATAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R3HR30

Antitoxin

Source ID Structure
AlphaFold DB A0A6B1XRC3

References