Detailed information of TA system TA171002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 44296..44915
• Replicon: plasmid pSF1
• Accession: NZ_CP060801
• Organism: Enterococcus faecalis strain SF28073

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: R3H4V2
• Locus tag: H9Q64_RS00225
• Protein ID: WP_000241511.1
• Coordinates: 44553..44915 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3H5D1
• Locus tag: H9Q64_RS00220
• Protein ID: WP_000245205.1
• Coordinates: 44296..44559 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9Q64_RS00205	39556..40869	+	1314	WP_002383787.1	hypothetical protein	-
H9Q64_RS00210	41250..41891	+	642	WP_000406546.1	hypothetical protein	-
H9Q64_RS00215	43142..43798	+	657	WP_000411487.1	hypothetical protein	-
H9Q64_RS00220	44296..44559	+	264	WP_000245205.1	PbsX family transcriptional regulator	Antitoxin
H9Q64_RS00225	44553..44915	+	363	WP_000241511.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H9Q64_RS00230	44980..45942	-	963	WP_002367770.1	LacI family DNA-binding transcriptional regulator	-
H9Q64_RS00235	45944..47383	-	1440	WP_002383789.1	sucrose-6-phosphate hydrolase	-
H9Q64_RS00240	47574..49505	+	1932	WP_002367772.1	sucrose-specific PTS transporter subunit IIBC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	ant(6)-Ia / aph(3')-III / erm(B)	prgB/asc10	1..96486	96486

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13585.78 Da
• Isoelectric Point: 9.6107

>T171002 WP_000241511.1 NZ_CP060801:44553-44915 [Enterococcus faecalis]
MVKVPHQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVIVAPISSTKRNYPLYVSINPSYGMKTSGKVLLDQLT
TIDYEARQCVFLETAHEKLIDELLLKVRTVFQKVNKTNKF

Nucleotide

• Length: 363 bp

>T171002 NZ_CP077812:4305029-4305397 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTTATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10059.46 Da
• Isoelectric Point: 5.1585

>AT171002 WP_000245205.1 NZ_CP060801:44296-44559 [Enterococcus faecalis]
MVMLSIKKWGNSNGLRLPKSVMEYLQIHTEDKVKITQEESNGKKRLIIEAVDSDNDLTIEQLFENYKEEKVHVTIQDLGN
AVGNEKW

Nucleotide

• Length: 264 bp

>AT171002 NZ_CP077812:4304811-4305032 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: R3H4V2

Antitoxin

• AlphaFold DB: R3H5D1