Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 505920..506556 Replicon chromosome
Accession NZ_CP060799
Organism Bacillus pumilus strain ONU 554

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag H9S87_RS02400 Protein ID WP_003214169.1
Coordinates 506206..506556 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A267X601
Locus tag H9S87_RS02395 Protein ID WP_012008995.1
Coordinates 505920..506201 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9S87_RS02375 502066..502671 - 606 WP_187704345.1 rhomboid family intramembrane serine protease -
H9S87_RS02380 502766..503131 + 366 WP_034663960.1 holo-ACP synthase -
H9S87_RS02385 503292..504308 + 1017 WP_095286222.1 outer membrane lipoprotein carrier protein LolA -
H9S87_RS02390 504445..505626 + 1182 WP_106038585.1 alanine racemase -
H9S87_RS02395 505920..506201 + 282 WP_012008995.1 hypothetical protein Antitoxin
H9S87_RS02400 506206..506556 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
H9S87_RS02405 506674..507549 + 876 WP_045210228.1 RsbT co-antagonist protein RsbRA -
H9S87_RS02410 507509..507877 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
H9S87_RS02415 507880..508281 + 402 WP_034663954.1 anti-sigma regulatory factor -
H9S87_RS02420 508292..509299 + 1008 WP_095286219.1 PP2C family protein-serine/threonine phosphatase -
H9S87_RS02425 509359..509688 + 330 WP_034663950.1 anti-sigma factor antagonist -
H9S87_RS02430 509685..510173 + 489 WP_034663948.1 anti-sigma B factor RsbW -
H9S87_RS02435 510139..510927 + 789 WP_034663946.1 RNA polymerase sigma factor SigB -
H9S87_RS02440 510927..511526 + 600 WP_034663944.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T171001 WP_003214169.1 NZ_CP060799:506206-506556 [Bacillus pumilus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T171001 NZ_CP077812:c3903474-3903091 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10894.27 Da        Isoelectric Point: 5.2726

>AT171001 WP_012008995.1 NZ_CP060799:505920-506201 [Bacillus pumilus]
MSESSATNEMKIRLPETLMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT171001 NZ_CP077812:c3903716-3903474 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCACGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A267X601

References